老鼠簕生物碱A及其衍生物对四氯化碳致大鼠肝纤维化的保护作用  

Protective effect of Ilicifoliosids alkaloid A and its derivatives on hepatic fibrosis induced by carbon tetrachloride in rats

作　　者：卢顺玉[1] 祁平[1] 梁颖娥[1] 陈小英[1] 蔡丽萍[1] 周丽娟[1] 刘林[1] 林军[1] 

机构地区：[1]广西医科大学,广西南宁530021

出　　处：《时珍国医国药》2013年第2期310-311,共2页Lishizhen Medicine and Materia Medica Research

基　　金：广西壮族自治区自然科学基金(No.2009AM4019);广西研究生教育创新计划资助项目(No.2011105981007M175)

摘　　要：目的观察老鼠簕生物碱A及其衍生物对四氯化碳所致大鼠肝纤维化的保护作用,并探讨其疗效机制。方法用四氯化碳(Carbon tetrachloride,CCl4)-花生油溶液腹腔注射8周建立大鼠肝纤维化模型,将造模成功的大鼠随机分为5组,分别为模型对照组、阳性对照组(秋水仙碱0.4 mg.kg-1)、HBOA组(100 mg.kg-1)、TC-2组(100 mg.kg-1)、TC-3组(100 mg.kg-1)。用药物干预4周,检测肝纤维化大鼠血清天冬氨酸转氨酶(AST)活性以及肝组织匀浆中超氧化物歧化酶(SOD)活性和丙二醛(MDA)含量。结果 HBOA、TC-2、TC-3均能显著降低血清AST的活性(P<0.05),TC-3能降低肝匀浆MDA的含量(P<0.05),同时提高SOD的活性(P<0.05);HBOA可提高SOD活性(P<0.05)。结论HBOA及其衍生物对CCl4致大鼠肝纤维化具有一定的保护作用,其保护机制可能与清除自由基、抗脂质过氧化作用有关。ObjectiveTo study the protective effect of Ilicifoliosids alkaloid A and its derivatives on hepatic fibrosis induced by carbon tetrachloride（CCl4） in rats. MethodsThe rats were given CCl4-peanut oil by intraperitoneal injection for 8 weeks,then the successful model rats were randomly divided into 5 groups,respectively：the model group,the positive group（150 mg·kg-1）,the HBOA group（200 mg·kg-1）,the TC-2 group（200 mg·kg-1） and the TC-3 gro-up（200 mg·kg-1）.AST in serum and MDA 、SOD in liver tissue were measured in the model of hepatic fibrosis induced by CCl4 in rats. ResultsCompared with the model group,the activity of serum AST in the HBOA、TC-2、TC-3 group decreased significantly,MDA in liver tissue was low and the level of SOD in liver tissue was increased in the TC-3 group;the HBOA group elevated the level of SOD in liver tissue. ConclusionIlicifoliosids alkaloid A and its derivatives can afford a better protective effect against carbon tetrachloride-induced hepatic fibrosis in rats.

关 键 词：老鼠簕生物碱A及其衍生物 肝纤维化 四氯化碳 

分 类 号：R285.5[医药卫生—中药学]