叠氮胸苷体外增强紫杉醇对宫颈癌细胞化疗敏感性的实验研究  

Effect of Azidothymidine' s enhancing chemotherapy sensitivity of taxol on human cervical Carcinoma Hela Cell Line in vitro

作　　者：邓守恒[1] 蔡晓军[1] 曹风军[1] 李芳[1] 雷金华[1] 陈萍[1] 

机构地区：[1]湖北医药学院附属人民医院,湖北十堰442000

出　　处：《时珍国医国药》2013年第2期334-336,共3页Lishizhen Medicine and Materia Medica Research

基　　金：湖北省教育厅科研项目(No.Q20092402);湖北医药学院附属人民医院基金资助项目(十人医字[2005]86号)

摘　　要：目的观察叠氮胸苷(AZT)体外增强紫杉醇对宫颈癌HeLa细胞化疗敏感性的作用,并探讨可能的作用机制。方法实验分为空白对照组、紫杉醇组(培养液中紫杉醇终浓度为360 nmol/L),AZT组(培养液中AZT终浓度为400μmol/L)、联合组(培养液中AZT终浓度为400μmol/L,紫杉醇浓度为360 nmol/L),各组作用24 h,MTT法检测细胞增殖抑制率,金氏公式评价二药的协同效果;流式细胞仪检测细胞凋亡率及周期分布;端粒重复序列扩增法联合酶联免疫吸附法(TRAP-ELISA)检测端粒酶活性;RT-PCR法检测细胞hTERTmRNA水平。结果 AZT、紫杉醇均可抑制HeLa细胞增殖、诱导细胞凋亡、增加S期细胞数量、降低细胞端粒酶活性和hTERTmRNA水平,二者联合应用呈协同效应(P<0.01)。结论 AZT能够增强紫杉醇对宫颈癌HeLa细胞的化疗敏感性,这种作用与AZT能够抑制反转录酶活性和端粒酶活性有关。ObjectiveThe aim was to observe the effect of Azidothymidine,s enhancing chemotherapy sensitivity of taxol on human cervical Carcinoma Hela Cell Line in vitro and to explore its possible mechanism. MethodsThere were four groups： Control group,Azidothymidine（AZT） group（HeLa cells were pretreated by 400μmol/LAZT）,Taxol group（HeLa cells were pretreated by360nmol/L Taxol）,AZT/Tazol group（HeLa cells were pretreated by360nmol/L Taxol and 400μmol/LAZT）,after treatment for 24h,MTT method was used to determine the proliferative inhibition rate and Jin,sformula was used to assess the synergistic effects between two drugs.Cell apoptosis and phase were detected by the flow cytometry.Telomerase activity was measured by polymerase chain reaction enzyme-linked immunoassay;The expression of hTERT mRNA was analyzed by reverse transcriptase polymerase chain reaction. ResultsAZT、Taxol alone and combination could inhibite cell proliferation、induce cell apoptosis、increase S phase cells 、 decrease Telomerase activity and down-regulate the expression of hTERT mRNA in HeLa cells.The effect of Taxol combinated with AZT was better than that of Taxol 、AZT alone（P0.01）. ConclusionAZT could enhance chemotherapy sensitivity of taxol on human cervical Carcinoma Hela Cell Line in vitro,which was related to the inhibition of reverse transcriptase and reduction of telomerase activity and hTERT mRNA expression on tumor cells.

关 键 词：宫颈癌癌 叠氮胸苷 紫杉醇 化疗 体外 

分 类 号：R962[医药卫生—药理学]