荧光法研究PSt-b-PCA-b-PSt与牛血清白蛋白的相互作用  

Fluorescence Research on the Interaction Between PSt-b-PCA-b-PSt and Bovine Serum Albumin

作　　者：王千[1] 王峥[1] 邹斯婷[1] 

机构地区：[1]湖北大学化学化工学院,湖北武汉430062

出　　处：《胶体与聚合物》2013年第1期36-39,共4页Chinese Journal of Colloid & Polymer

基　　金：湖北省教育厅科研项目(D20091006)

摘　　要：采用可逆加成-断裂链转移聚合方法(RAFT)合成了聚苯乙烯-b-聚肉桂酸-b-聚苯乙烯两亲性三嵌段聚合物(PSt-b-PCA-b-PSt),采用荧光光谱法研究了PSt-b-PCA-b-PSt与牛血清白蛋白的相互作用。结果表明,PSt-b-PCA-b-PSt在选择性溶剂中自组装形成半径约为50nm的胶束;根据Stern-Volmer方程计算得到双分子猝灭过程速率常数、结合常数和结合数,表明三嵌段聚合物PSt-b-PCA-b-PSt与牛血清白蛋白之间发生了较强的静态荧光猝灭作用。An amphiphilic triblock copolymer PSt-b-PCA-b-PSt was synthesized via reversible addition-fragmentation chain transfer polymerization （RAFT）. The binding interaction of PSt-b-PCA-b-PSt colloidal spheres with bovine serum albumin （BSA） was investigated by fluorescence spectra technique. The results showed that the triblock copolymer self-assembled into micelles with the size of about 50nm in selective solution. Quenching constants and the number of binding sites were determined according to the Stem Volmer equation. Mechanism discussion results indicated that the triblock copolymer PSt-b-PCA-b-PSt spheres caused the fluorescence quenching of BSA by a static quenching procedure.

关 键 词：三嵌段聚合物 牛血清白蛋白 荧光光谱 

分 类 号：O64[理学—物理化学]