阿霉素诱导兔心衰模型的建立与评价  

Establishment and Evaluation of Doxorubicin Induced Rabbit Heart Failure Model

作　　者：师幸伟 程冕[2] 白融[3] 阮磊[2] 闫杰 侯宏伟 李秀琪 宋玉娥[3] 

机构地区：[1]长江航运总医院心内科,湖北武汉430000 [2]华中科技大学同济医学院附属同济医院综合科,湖北武汉430000 [3]华中科技大学同济医学院附属同济医院心内科,湖北武汉430000 [4]武汉第六医院心内科,湖北武汉430000 [5]鄂州中心医院心内科,湖北鄂州436000

出　　处：《动物医学进展》2013年第3期44-47,共4页Progress In Veterinary Medicine

基　　金：湖北省自然科学基金资助项目(2009CDB067)

摘　　要：探讨静脉注射阿霉素(ADR)建立兔心衰动物模型的可行性。将日本大耳白兔32只,随机分成模型组1、2和对照组;模型组1注射ADR 1mg/kg,每周2次,共8周;模型组2注射ADR 2mg/kg,每周1次,共8周;对照组注射相同体积9g/L NaCl注射液。于停药3周后进行心脏彩超检查,并进行心肌组织病理切片观察其组织学改变。与对照组相比,模型组动物左室舒张末期容积明显增大,左室射血分数明显降低(P<0.05),病理检查符合扩张性心肌病样改变;模型组间无统计学差别。常规剂量多次静脉注射ADR可显著损伤心肌,造成心脏舒缩功能下降,是建立动物心衰模型的一种简便可行的方法。低浓度多次注射更有利于制模期间的观察。To investigate the feasibility of the rabbit heart failure animal model by rejecting mtravenous adriamycin （ADR）. 32 Japanese white rabbits were randomly divided into Model group 1, 2 and Control group, Model group 1： injection of ADR 1 mg/kg, 2 times in a week, for 8 weeks; model group 2：injection of ADR 2 mg/kg,once in a week, for 8 weeks; control group, injected with the same volume of 9 g/L NaCI injection. After three weeks of withdrawal, rabbits in the three groups were measured by color doppler ultrasound; and the myocardial tissue sections were examined for pathological lesions. Compared with the control group, the left ventricular end-diastolic volume of model groups increased significantly, LVEF was significantly 1Qwer （P〈0.05）, conforming pathological changes of cardiomyopathy, and the two model group were not statistically different. By conventional dose multiple injection, ADR could injure significantly myocardial organization and reduce cardiac ventricular and systolic functions. This is a simple and practical method to establish animal heart failure model. Low concentrations of multiple injections were more conducive in observation of model establishment.

关 键 词：阿霉素 兔 心力衰竭 

分 类 号：R331[医药卫生—人体生理学]