山西省瓜类作物根茎部镰刀菌的分离与鉴定  

Isolation and Identification of Fusarium from Roots of Cucurbits Crops in Shanxi Province

作　　者：袁微微[1] 李新凤[1] 高俊明[1] 王建明[1] 郝晓娟[1] 贺运春[1] 

机构地区：[1]山西农业大学农学院,山西太谷030801

出　　处：《山西农业科学》2013年第3期246-250,258,共6页Journal of Shanxi Agricultural Sciences

基　　金：国家自然科学基金项目(31000873);山西省自然科学基金项目(2006011080);山西省科技攻关项目(20120311019-3);山西农业大学科技创新基金项目(412556)

摘　　要：为明确山西省瓜类作物上镰刀菌的种类,自山西省不同地区采集了具有萎蔫症状和健康的瓜类作物标本,并采用常规组织分离法对其根茎部镰刀菌进行了分离、纯化,共分离到353株镰刀菌菌株;经形态学鉴定,其分属尖孢镰刀菌(F.oxysporum)、芬芳镰刀菌(F.redolens)、腐皮镰刀菌(F.solani)、轮枝镰刀菌(F.verticillioides)、层生镰刀菌(F.proliferatum)、禾谷镰刀菌(F.graminearum)、半裸镰刀菌(F.semitectum)和锐顶镰刀菌(F.acuminatum)8个种,其中,尖孢镰刀菌为优势菌群,占镰刀菌总量的65.03%;并对这8个种进行了形态学描述。To know the Fusarium species of cucurbits crops in Shanxi, cucurbits crops with and without wilt symptoms were collected from various areas in Shanxi and from which the Fusarium strains were isolated and purificated by the conventional tissue method. The total 353 isolates belonging to 8 species were isolated from the roots of the samples. Based on the morphological identification, which were identified as Fusarium oxysporum, F. redolens, F. solani, F. verticillioides, E proliferatum, F. graminearum, F. semitectum, and F. acuminatum respectively. The predominant species was F. oxysporum accounting for 65.03% of the total Fusarium. This paper described the morphology of these eight species.

关 键 词：瓜类作物 镰刀菌 分离纯化 形态学鉴定 山西省 

分 类 号：S436.5[农业科学—农业昆虫与害虫防治]