5-氮杂-2'-脱氧胞苷对HepG2肝癌细胞生物学行为的影响  

The effects of 5-Aza-2′-deoxyetidine on the biological behaviors of human hepatocarcinoma cell line HepG2

作　　者：刘瑶[1] 罗耀玲[2] 黄文峰[1] 王小农[3] 黄才斌[1] 

机构地区：[1]赣南医学院第一附属医院消化内科,江西赣州341000 [2]赣南医学院第一附属医院临床医学研究中心,江西赣州341000 [3]赣南医学院第一附属医院普外科,江西赣州341000

出　　处：《广东医学》2013年第4期505-508,共4页Guangdong Medical Journal

基　　金：国家自然科学基金资助项目(编号:81000908);江西省自然科学基金资助项目(编号:2010GQY0036)

摘　　要：目的研究5-氮杂-2'-脱氧胞苷(5-Aza-CdR)对人肝癌细胞株HepG2生物学行为的影响及其机制。方法用浓度为50μmol/L的5-Aza-CdR处理HepG2细胞72 h后,流式细胞仪分析HepG2细胞周期的分布和凋亡的情况;transwell实验检测细胞侵袭能力;RT-PCR检测肝癌中失活的抑癌基因SIAH1 mRNA表达量的变化。并与未经任何药物处理的正常HepG2细胞相比。结果与正常HepG2细胞相比,5-Aza-CdR处理后的HepG2细胞周期阻滞于S期(P<0.05),细胞早期凋亡率增加(P<0.05),且细胞体外侵袭力减弱(P<0.05),SIAH1基因的表达水平明显上调(P<0.05)。结论 5-Aza-CdR可能通过上调SIAH1的表达,调控细胞周期并诱导细胞凋亡,同时抑制HepG2细胞体外侵袭能力。Objective To investigate the effects and mechanism of 5 - Aza - 2＇ - deoxyetidine （5 - Aza - CdR） on the biological behaviors of human hepatocarcinoma cell line HepG2. Methods The HepG2 ceils were treated with 50μmoL/L 5 - Aza - CdR for 72h. The cell cycle and apoptosis were assessed by flow cytometry （FCM） with annexin - FITC/PI staining, while the invasive ability was examined by using transwell invasion assay. The mRNA expression oftumor suppressor gene SIAH1 was detected by using reverse transcription polymerase chain reaction （RT -PCR） before and after 5 - Aza - CdR treatment. Results After 5 - Aza - CdR treatment, significant increase of S phase cell count wasobserved with FCM analysis （ P 〈 0. 05 ） , indicating cell cycle blockage in HepG2 ceils ; meanwhile, significant elevation of apoptosis rate, reduction in invasive activity and up - regulation of SIAH1 were also revealed （P 〈 0. 05）. Conclusion The 5 - Aza - CdR regulates cell cycle and induces apoptosis, as well as inhibits the cell invasion, through up - regulation of the expression of SIAH1.

关 键 词：5-氮杂-2'-脱氧胞苷 肝癌 细胞周期 凋亡 SIAH1 

分 类 号：R977.9[医药卫生—药品]