功能单体对分子烙印手性固定相手性拆分能力的影响  

Effect of Functional Monomer on Chiral Separation Ability of Molecular Imprinted Chiral Stationary Phase

作　　者：王进防[1] 周良模[1] 刘学良[1] 王清海[1] 朱道乾[1] 

机构地区：[1]中国科学院大连化学物理研究所,大连116012

出　　处：《化学学报》2000年第3期351-355,共5页Acta Chimica Sinica

基　　金：国家自然科学基金(29575212)资助项目

摘　　要：系统考察了功能单体对非共价分子烙印手性固定相手性分离能力的影响,发现在非共价分子烙印手性固定相的制备中,功能单体与烙印分子之间存在着匹配性.丙烯酰胺可以与氨基酸衍生物的酰胺基团形成较强的氢键作用,碱性功能单体2-乙烯基吡啶则与其羧基形成较强的离子作用,两者的协同作用使复合功能单体丙烯酰胺+2-乙烯基吡啶对于氨基酸衍生物具有良好的烙印效果.竞争溶剂乙酸对样品与分子烙印固定相间的非共价作用力有较大的影响,增加流动相中的竞争溶剂乙酸的含量,将减弱分子烙印手性固定相与样品的酰胺键和羧基的氢键及离子作用,导致对样品的容量因子、手性选择性α及分离度f/g的减小.The effect of functional monomer on chiral separation ability of the non - covalent molecular imprinted chiral stationary phase was investigated systematically. It was found that there was compatibleness between the functional monomer and the template molecules. Strong hydrogen bond and ionic interaction could be formed between the amide group and carboxyl group in the amino acid derivatives and acrylamide and 2 -vinylpyridine, which made the imprinting effect of the combined functional monomers AM + 2 - VP more evident. The competing solvent acetic acid showed great influence on the non - covalent interaction between the analyte and the functional groups of molecular imprinted polymer (MIP). Ionic interaction and hydrogen bonding between amide group and carboxyl group in the analyte and the functional groups of MIP were weakened by increasing the concentration of the competing solvent acetic acid, therefore both the capacity factor, chiral selectivity factor and resolution decreased.

关 键 词：分子烙印聚合物 氨基酸衍生物 手性拆分 HPLC 

分 类 号：O658[理学—分析化学]