毛细管超薄池光度吸收测量新技术  

A New Absorption Measurement Technique for a Capillary Super - Thin Cell

作　　者：许崇峰[1] 张素艳[1] 樊惠芝[1] 庄峙厦[2] 赵一兵[2] 杨芃原[1] 

机构地区：[1]复旦大学化学系,上海200433 [2]厦门大学教育部分析科学重点实验室,厦门361005

出　　处：《化学学报》2000年第8期1022-1026,共5页Acta Chimica Sinica

基　　金：国家自然科学基金(29875005);厦门大学分析科学教育部重点实验室基金资助项目

摘　　要：在毛细管超薄池光度检测中首次采用吸收光强测量新技术,显著提高了测量的灵敏度和改善了信噪比.在内径50μm的石英毛细管超薄池以及由石英光导纤维构成的十字交叉超薄光学吸收池上,分别测量了系列高锰酸钾溶液吸收光强.吸收光强与高锰酸钾溶液浓度之间呈现良好的线性关系.对于10μg/mL的高锰酸钾稀溶液,毛细管超薄池吸收光强测量的信噪比相对于吸光度测量有较大的改善.十字交叉池的光程约为40μm,容积约为0.02μL,具有较大的光通量,可望发展成为一种新型的电泳光度检测池.We report a new technique of absorption measurement for capillary super - thin cell in the first time. The new technique determines the absorbed intensity instead of the absorbance as conventional way. Theabsorbed intensity is measured for a capillary cell (i. d. 50μm) and a decussate optical super - thin cell(40μm) for serial KMnO4 solutions. High sensitivity and good S/N ratio are observed, and the calibrationcurves are fairy linear. For a 10μg/mL KMnO4 solution, the S/N ratio of absorbed intensity determination is about 10, which is better than that of absorbance determination in the capillary cell. This super- thin cell in the decussate configuration provides a novel type optical detection cell for capillary electrophoresis.

关 键 词：超薄池 高效毛细管电泳 吸收光强测量 光度检测 

分 类 号：O657.3[理学—分析化学]