1,3-丙二醇生物法生产中关键酶的研究进展  

Advances on the Key Enzymes of Microbial Production of 1,3-propanediol

作　　者：王飞[1] 邓文颖[1] 杨泽茜[1] 徐浪[1] 齐向辉[1] 

机构地区：[1]江苏大学食品与生物工程学院,江苏镇江212013

出　　处：《生物技术》2013年第1期93-96,共4页Biotechnology

基　　金：国家自然科学基金项目("宏基因组甘油脱水酶分子催化机制及分子改造";21006041);江苏大学基金项目("新型甘油脱水酶基因的克隆与高效表达";08JDG009;11A344;Y11A169)资助

摘　　要：目前,国内外对于1,3-丙二醇(1,3-propanediol,1,3-PD)的生产研究正在由化学法逐渐向生物法转变。该文着重介绍了生物法生产1,3-PD的生产菌株和生物合成途径,综述了关键酶的性质特点和基因克隆表达情况,对关键酶晶体结构的相关研究成果进行了介绍,进一步探讨了运用宏基因组技术和酶分子改造技术来获得新型高性能关键酶的方法,并展望了基因工程菌的应用前景,从而推动了对1,3-PD生产途径中关键酶的了解及1,3-PD的生产应用研究。In present, the research on the method of 1,3 -propanediol＇ s production was gradually changed from chemical to microbial do- mestic and overseas. In this article, biosynthetic pathway of 1,3 -propanediol by microorganism was introduced, properties of key enzymes and molecular cloning and expression were reviewed. At the same time,the crystal structures of key enzymes was introduced,and the tech- nology of metagenome and enzyme molecule modification to obtain new high performance key enzyme was further discussed. Finally, the ap- plication of gene engineering bacteria was prospected. This study may be helpful to understanding of the key enzymes and to application re- search of 1,3 - propanediol.

关 键 词：1 3-丙二醇 关键酶 克隆表达 晶体结构 宏基因组 分子改造 

分 类 号：Q814[生物学—生物工程]