N-乙酰半胱氨酸抑制大鼠肾草酸钙结石形成机制的研究  

Research the mechanism of N-acetyl cysteine inhibiting renal calcium oxalate stone formation in rats

作　　者：张雍偲[1] 刘春[1] 

机构地区：[1]山西医科大学第一医院泌尿外科,太原030001

出　　处：《临床泌尿外科杂志》2013年第3期222-225,共4页Journal of Clinical Urology

摘　　要：目的:探讨N-乙酰半胱氨酸(NAC)对大鼠肾草酸钙结石形成的影响及机制,为临床预防尿路结石提供理论依据。方法:将30只健康清洁成年雄性Wistar大鼠先在相同环境下适应性喂养1周,然后随机分为3组:A组(空白对照组)、B组(单纯诱石组)、C组(诱石+NAC干预组)。A组饮去离子水,B组饮1%乙二醇的去离子水,C组饮1%乙二醇去离子水,并给予NAC 100mg/(kg.d)灌胃。第4周处死大鼠,取出双肾,左肾纵向剖开,用10%甲醛固定,HE染色石蜡切片,在100、400倍光镜下观察肾组织草酸钙结晶沉积情况,并进行分级及评分。右肾皮质制成10%的匀浆,检测丙二醛(MDA)及超氧化物歧化酶(SOD)。全部实验数据通过SPSS17.0统计软件分析处理。结果:①肾脏结晶沉积情况分级及评分结果:与B组相比,C组的肾脏结晶沉积评分明显降低(P<0.05)。②MDA检测结果:与B组相比,C组的MDA含量降低,差异有统计学意义(P<0.05)。③SOD检测结果:与B组相比,C组的SOD含量增高,差异有统计学意义(P<0.05)。④A、B、C三组肾组织结晶等级评分与SOD含量的相关系数为-0.499(P<0.01),结晶评分与MDA含量的相关系数为0.592(P<0.01)。结论:NAC可以通过其抗氧化作用抑制大鼠肾草酸钙结石的形成。Objective：To study the effect and mechanism of N-acetyl cysteine（NAC） on renal calcium oxalate stone formation in rats, For clinical prevention urinary stones provide theoretical basis. Methods： At first, 30 health clean adult male Wistar rats adaptability feeding 1 week in the same environment, then randomly divided into 3 groups（n： 10） ：group A（Blank control group）, group B（stone forming group）, group C（stone forming＋NAC intervention group）, group A drink deionized water, group B drink 1% glycol in deionized water, group C drink 1% glycol in deionized water, and to give NAC100 mg/（kg · d） by lavage. After feeding for 4 weeks, remove double kidney, left renal opened longitudinally,with 10% formaldehyde fixed, HE dyeing paraffin section, the deposit condition of calcium oxalate crystals in nephridial tissue was observed and scored by 100, 400 times light-microscopy. Right renal cortex made 10% of slurry, detection of MDA and SOD. All the experimental data analysis by SPSS17.0 statistical software. Results：①Classification and score the deposit condition of calcium oxalate crystals in nephridial tissue： Compared with group B, group C of kidney crystal deposition score significantly decrease （P〈0.05） ; ②MDA test results： Compared with group B, group C of MDA content decreased, difference have statistical significance（P〈0. 05）; ③SOD test results： Compared with group B, group C of SOD content increased, difference have statistical significance（P〈0.05）; ④The correlation coefficient of score（deposit condition of calcium oxalate crystals in nephridial tissue） and SOD content is -0. 499（P〈0.01）, the correlation coefficient of score and MDA content is 0. 592（P〈0.01）. Conclusions： NAC can inhibiting renal calcium oxalate stone formation in rat through its antioxidation.

关 键 词：肾结石 N-乙酰半胱氨酸 超氧化物歧化酶 丙二醛 

分 类 号：R692.4[医药卫生—泌尿科学]