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作 者:李光华[1] 赵楠[1] 杨敏[1] 赵志芳[1] 罗彦[1] Osamu Shido
机构地区:[1]宁夏医科大学基础医学院生理学系,银川750004 [2]岛根大学医学部环境生理教研室
出 处:《第二军医大学学报》2013年第3期291-294,共4页Academic Journal of Second Military Medical University
基 金:国家自然科学基金(81060230);宁夏医科大学校级重点孵育科研项目(XZ2012005)~~
摘 要:目的探讨固定时间热暴露对大鼠胸主动脉血管收缩特性的影响及机制。方法选用雄性Wistar大鼠,随机分为对照组、随意时间热暴露组和固定时间热暴露组,环境温度24℃,热暴露温度为36℃,每天热暴露6h。经7d热暴露后,采用体温遥感技术监测大鼠体核温度变化;器官浴槽法测定大鼠胸主动脉对去甲肾上腺素(NA)的反应性;RT-PCR检测动脉血管内皮型一氧化氮合酶(eNOS)mRNA的表达水平及用试剂盒测定血浆中一氧化氮等离子体(NOx)NO2-和NO3-的含量变化。结果热暴露对大鼠的体核温度变化影响不大(P>0.05);与对照组相比,固定时间热暴露组大鼠胸主动脉血管对NA的反应性降低(P<0.01);与对照组和随意时间热暴露组相比,固定时间热暴露组eNOS mRNA相对表达水平及血浆中NOx的含量增加(P<0.05)。结论固定时间热暴露可改变大鼠胸主动脉血管的反应性,内皮细胞源性的NOS通路可能参与并诱导了血管舒张作用。Objective To investigate the effect of heat exposure during fixed time on the thoracic aorta contractile properties of rats and the related mechanism. Methods Male Wistar rats were randomly divided into control group, random- time heat exposure group and fixed-time heat exposure group (ambient temperature to 24℃;the two heat exposure groups were exposed to heat [36℃] 6 h daily). After 7 days of heat exposure, the body core temperature of rats was measured by telemetry technology. The response of isolated thoracic aorta to noradrenaline (NA) was determined by organ bath system. Expression of aorta eNOS mRNA and plasma NOx (NO2- and NO3- ) content were also examined. Results Heat exposure showed no significant effects on body core temperature of rats (P〉0. 05). Compared with control group, the fixed-time heat exposure group had significantly lowered thoracic aortic vascular reactivity to NA (P〈0.01). Compared with control group and random- time heat exposed group, the fixed-time heat exposure group had significantly increased eNOS mRNA expression and plasma NOx content (P〈0.05). Conclusion Fixed-time exposure to heat can change the reactivity of the thoracic aorta in rats, and vascular endothelial cell-derived NOS pathway may be involved in the vasodilatory effects.
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