中国南海黑乳海参共附生白色侧齿霉菌中的甾体类成分  

Steroids from fungus Engyodontium album associated with the South China Sea cucumber Holothuria nobilis Selenka

作　　者：宫俊[1] 汤华[1] 刘宝姝[1] 周巍[1] 孙鹏[1] 李玲[1] 张文[1] 

机构地区：[1]第二军医大学药学院海洋药物研究中心,上海200433

出　　处：《第二军医大学学报》2013年第3期310-314,共5页Academic Journal of Second Military Medical University

基　　金：国家自然科学基金(30873200;41176125);上海浦江人才计划(PJ2008)~~

摘　　要：目的对中国南海黑乳海参共附生白色侧齿霉菌Engyodontium album的次生代谢产物进行研究。方法运用硅胶柱色谱、Sephadex LH-20凝胶柱色谱、高效液相色谱等分离手段对白色侧齿霉菌Engyodontium album的乙酸乙酯提取物进行分离纯化,根据现代波谱技术结合文献报道进行结构鉴定。结果共分离得到5个化合物,分别鉴定为:(22E,24R)-5α,8α-过氧化麦角甾-6,22-二烯-3β-醇(1)、(22E,24R)-麦角甾-5,7,22-三烯-3β-醇(2)、(22E,24R)-麦角甾-7,22-二烯-3β,5α,6β-三醇(3)、3β,5α-二羟基-(22E,24R)-麦角甾-7,22-二烯-6-酮(4)、3β,5α-二羟基-6β-甲氧基-麦角甾-7,22-二烯(5)。结论本研究是对黑乳海参共附生白色侧齿霉菌次生代谢产物的首次报道,这5个化合物均为首次从白色侧齿霉菌Engyodontium album中分离得到。Objective To investigate the secondary metabolites of fungus Engyodontium album （E. album） associated with the South China Sea cucumber Holothuria nobilis （H. nobilis） Selenka. Methods The EtOAc extract of E. album was purified by repeated column chromatography on silica gel, Sephadex LH-20, and high-performance liquid chromatography （HPLC）. Structures of the obtained compounds were determined by spectroscopic analysis and comparison with previously reported data. Results Five compounds were isolated and their structures were determined as： （22E, 24R）-5a,8a-epidioxy- ergosta-6,22-dien-3β-ol （1）, （22E, 24R）-ergosta-5,7,22-trien-3β-ol （2）, （22E, 24R）-ergosta-7,22-dien-3β, 5a, 6β-triol （3）, 3β, 5a-dihydroxy-（22E,24R）-ergosta-7,22-dien-6-one （4）, 3β,5a-dihydroxy-6β-methoxy-ergosta-7,22-dien （5）. Conclusion This is the first report on the secondary metabolites of E. album associated with the sea cucumber H. nobilis Selenka, and the five compounds have been reported for the first time from the fungus E. album.

关 键 词：海参纲 黑乳海参 白色侧齿霉菌 甾类 

分 类 号：R931.77[医药卫生—生药学]