参芪复方对GK大鼠主动脉血管紧张素Ⅱ1型受体mRNA表达的影响  

Effects of Shenqi Compound on the mRNA Expression of AT1R in the Aorta of GK Rats

作　　者：庄灿[1] 谢春光[2] 陈敏[2] 刘桠[2] 高泓[2] 

机构地区：[1]柳州市柳铁中心医院中医科,广西545007 [2]成都中医药大学附属医院内分泌科,成都610075

出　　处：《中国中西医结合杂志》2013年第3期351-355,共5页Chinese Journal of Integrated Traditional and Western Medicine

基　　金：国家自然科学基金资助项目(No.30772806)

摘　　要：目的观察参芪复方对GK(Goto-Kakizaki)大鼠大血管病变主动脉血管紧张素Ⅱ1型受体(angio-tensinⅡtype1 receptor,AT1R)mRNA表达的影响。方法 67只GK大鼠随机分为GK组(18只)、模型组(16只)、阿托伐他汀组(17只)及参芪复方组(16只),另设正常Wistar对照组(18只)。以L-NAME0.10mg/(mL·d)加入大鼠饮用水中复制糖尿病大血管病变模型。除正常Wistar对照组外,其他4组均喂饲高脂饲料。阿托伐他汀组及参芪复方组分别按1.60mg/(kg·d)、1.44g/(kg·d)灌胃相应药物,均每天1次,连续35天。采用葡萄糖氧化酶法每周测定血糖1次;给药5周后,夹心酶联免疫吸附法测定甘油三酯(TG)及总胆固醇(TC)水平,放射免疫法检测血清血管紧张素Ⅱ(angiotensinⅡ,AngⅡ)水平,实时定量聚合酶链反应(RT-PCR)检测主动脉AT1R mRNA表达。结果给药4周末阿托伐他汀组和参芪复方组血糖水平均较本组给药前明显降低(P<0.05),且参芪复方组明显低于模型组同期(P<0.05)。模型组TC、TG、血清AngⅡ及主动脉AT1R mRNA水平均明显高于正常Wistar对照组(P<0.01)。给药5周后,阿托伐他汀组和参芪复方组TC、TG、AngⅡ及AT1R mRNA水平明显低于模型组(P<0.01,P<0.05)。阿托伐他汀组AT1R mRNA明显低于参芪复方组(P<0.05)。结论参芪复方可降低GK大鼠早期大血管病变模型的血糖、血脂,减少血清AngⅡ含量及主动脉AT1R mRNA表达。AT1R可能是参芪复方治疗糖尿病大血管病变的有效靶点之一。Objective To observe the effects of Shenqi Compound （SQC） on the mRNA expres- sion of angiotensin Ⅱ type 1 receptor （AT1 R） in the aorta of Goto-Kakizaki （GK） rats. Methods Totally 67 GK rats were randomly divided into 5 groups, i.e., the GK group （n =18）, the model group （n =16）, the atorvastatin group （n =17）, and the SQC group （n =18）. The diabetic macrovascular disease mode （n =16）. Another a normal control group was set up was prepared by adding L-NAME （at the daily dose of 0.10 mg/mL） in drinking water for GK rats. GK rats, except those in the normal control group were fed with high fat diet. Atorvastatin （at the daily dose of 1.60 mg/kg） and SQC （ at the daily dose of 1.44 g/kg） were respectively administered by gastrogavage, once daily for 35 successive days. The blood glucose was determined by glucose oxidase method once per week. After 5-week medication, the contents of tri- glyceride （TG） and total cholesterol （TC） were determined by ELISA. The serum concentrations of angio- tensin Ⅱ （Ang Ⅱ ） were determined by RIA. The mRNA expression of AT1R in the aorta was determined by real-time quantitative reverse transcriptase PCR （RT-PCR）. Results The blood glucose level was obviously lower in both the atorvastatin group and the SQC group after 4 weeks of medication （P 〈0.05）. Besides, it was significantly lower in the SQC group than in the model group by the end of the 4th week（P 〈0.05）. The concentrations of TG, TC and serum Ang Ⅱ , and the mRNA expression of AT1R in the a- orta were significantly higher in the model group than in the normal control group （P 〈0.01 ）. After 5-week medication, the concentrations of TG, TC and serum Ang Ⅱ , and the mRNA expression of AT1R in the a- orta were significantly lower in the atorvastatin group and the SQC group than in the model group （P 〈0.01, P 〈0.05）. The mRNA expression of AT1R was significantly higher in the SQC group than in the atorvastatin group （P〈0.05）. Conc

关 键 词：参芪复方 糖尿病大血管病变 血管紧张素Ⅱ 血管紧张素Ⅱ1型受体 

分 类 号：R285.5[医药卫生—中药学]