银杏黄酮苷元对氧化型低密度脂蛋白诱导的人主动脉内皮细胞氧化损伤的影响  

Effects of Ginkgo Glavone Aglycone on Oxidized LDL Induced Oxidative Injury of Human Aortic Endothelial Cells

作　　者：王玮玮[1,2] 何艳[1] 刘兴德[1] 

机构地区：[1]贵阳医学院附属医院心血管内科,贵阳550004 [2]山东省德州市人民医院心血管内科,山东253014

出　　处：《中国中西医结合杂志》2013年第3期392-396,共5页Chinese Journal of Integrated Traditional and Western Medicine

基　　金：社会发展领域科技攻关项目(No.T2010-9)

摘　　要：目的观察银杏黄酮苷元(Ginkgo flavone aglycone,GA)对氧化型低密度脂蛋白(oxidized low-den-sity lipoprotein,ox-LDL)诱导的人主动脉内皮细胞(human aortic endothelial cells,HAECs)氧化应激损伤的保护作用及其机制。方法体外培养HAECs,分为6组,即空白对照组、ox-LDL组、VitE组以及GA30、60、90mg/L组。除空白对照组外,其余5组均加入ox-LDL150mg/L复制氧化损伤模型;GA30、60、90mg/L组分别给予相应剂量GA干预;VitE组给予VitE200μmol/L干预。采用MTT法检测细胞存活率;CM-H2DCFDA荧光探针测定细胞内活性氧(ROS)含量;酶联免疫吸附法检测NADPH氧化酶含量;硫代巴比妥酸法检测丙二醛(MDA)水平;Griess reagent法测定一氧化氮(NO)含量;黄嘌呤氧化酶法测定超氧化物歧化酶(SOD)的含量。结果与空白对照组(100.00%)比较,ox-LDL组(70.68%)细胞存活率明显降低(P<0.05);VitE组、GA30mg/L组、GA60mg/L组细胞存活率分别为88.95%、83.25%、94.93%,均明显高于ox-LDL组(P<0.05),以60mg/L GA作用更强。与空白对照组比较,ox-LDL组细胞内ROS、MDA及NADPH氧化酶水平增加,NO含量及SOD活性降低,差异均有统计学意义(P<0.05);与ox-LDL组比较,GA30mg/L组、GA60mg/L组及VitE组细胞内ROS、MDA及NADPH氧化酶水平降低,NO含量及SOD活性增加,差异均有统计学意义(P<0.05),以60mg/L GA作用更强。结论 GA可明显抑制ox-LDL诱导的ROS合成,降低MDA水平,升高NO。其作用机制可能与SOD活性增加及NADPH氧化酶活性降低有关。Objective To observe the effects of ginkgo flavone aglycone （GA） on oxidized low- density lipoprotein （ox-LDL） induced oxidative injury of human aortic endothelial cells （HAECs） and its mechanisms. Methods HAECs were in vitro cultured. Then they were divided into 6 groups, i.e., the ve- hicle control group, the ox-LDL group, the GA 30 mg/l_ group, the GA 60 mg/L group, the GA 90 mg/L group, and the Vit E group. The oxidative injury model was duplicated in the rest 5 groups by adding 150 mg/L ox-LDL except the vehicle control group. GA was added as intervention at corresponding dose to the GA 30 mg/L group, the GA 60 mg/L group, and the GA 90 mg/L group. Vit E at 200 ~mol/L was ad- ministered to those in the Vit E group. The survival rate of HAECs was detected by M＇l-r. The contents of reactive oxygen species （ROS） in HAECs were determined by CM-H2DCFDA fluorescent probe. The con- tents of NADPH oxidase were detected by ELISA. The levels of malondialdehyde （MDA） were measured by thiobarbituric acid （TBA） test. The contents of nitric oxide （NO） were determined by Griess reagent method. The contents of superoxide dismutase （SOD） were detected by xanthine oxidase method. Results Compared with the vehicle control group （100.00%）, the cell survival rate in the ox-LDL group （70.68%） obviously decreased （P 〈0.05）. The cell survival rate was 88.95% in the VitE group, 83.25%in the GA 30 mg/L group, and 94.93% in the GA 60 mg/L group, obviously higher than that of the ox-LDL group （70.68%, P 〈0.05）. The optimal effects were shown in the GA 60 mg/L group. Compared with the vehicle control group, the contents of ROS, MDA, and NADPH oxidase increased, the contents of NO and the SOD activity decreased in the ox-LDL group, showing statistical difference （P 〈0.05）. Compared with the ox-LDL group, the contents of ROS, MDA, and NADPH oxidase decreased, the NO content and the SOD activity increased in the GA 30 mg/L group, the GA 60 mg/L group, and the Vit E group, showing

关 键 词：银杏黄酮苷元 内皮细胞 氧化型低密度脂蛋白 氧化应激 

分 类 号：R285[医药卫生—中药学]