结合重金属前后浮萍的红外光谱比较  

Comparison of Infrared Spectroscopy between Native and Binding Metals' Phytoplankton

作　　者：韩润平[1] 李建军[1] 杨贯羽[1] 鲍改玲[1] 

机构地区：[1]郑州大学化学化工学院,郑州450052

出　　处：《光谱学与光谱分析》2000年第4期489-491,共3页Spectroscopy and Spectral Analysis

基　　金：河南省科技攻关资助项目!(981160416)

摘　　要：对结合ZnSO4前后浮萍的红外光谱图进行了比较。结合Zn2 +后 ,3 40 0cm- 1 附近的羟基峰发生了位移 (Δν =83cm- 1 ) ,且吸光度下降 ,1 65 0和 1 5 4 0cm- 1 处的蛋白质特征峰的吸光度也下降 ,而 62 0cm- 1 处峰的吸光度随ZnSO4浓度的升高显著增大。因此羟基在结合Zn2 +时起重要作用 ,结合ZnSO4后细胞壁的结构发生了变化 ,结合ZnSO4时物理吸附与化学吸附共同起作用。Infrared spectroscopy of native and metalbinding duckweed was compared.After binding metals,the absorbance peak around 3 400 cm -1 ,which reflects OH group stretching vibration,had been moved about 83 cm -1 ,and its absorbance was decreased.The absorbance of the dominating bands near 1 650 and 1 540 cm -1 ,the characteristic IR absorption of protein,was decreased.Therefore,the peak absorbance at 620 cm -1 was increased significantly by exposure to high concentration of ZnSO 4 solution.So we concluded that OH group play a important role in binding zinc cation and the structure of cell walls had changed due to zinc existing.Furthermore,the process of binding metals by duckweed is physical adsorption and chemical adsorption simultaneously.

关 键 词：浮萍 红外光谱 硫酸锌 生物吸附 重金属离子 

分 类 号：Q67[生物学—生物物理学]