HMGB1及TLR2、TLR4在类风湿关节炎中的表达及意义  

Expression and significance of HMGB1 and TLR2/TLR4 in rheumatoid arthritis

作　　者：米亚英[1] 杨丽丽 孙利平[3] 

机构地区：[1]大同大学医学院免疫学研究所,山西大同037009 [2]山西省中西医结合医院检验科,山西太原030013 [3]山西省大同市第五人民医院免疫风湿科,山西大同037006

出　　处：《基础医学与临床》2013年第4期476-479,共4页Basic and Clinical Medicine

摘　　要：目的探讨HMGB1及TLR2、TLR4在类风湿关节炎患者中的表达。方法选取活动期类风湿关节炎患者29例,非活动期20例及对照者18例。用酶联免疫吸附试验(ELISA)检测血清HMGB1表达,用多色免疫荧光流式细胞术(FCM)检测外周血单核细胞表面TLR2和TLR4的表达率和蛋白相对含量。结果活动期RA患者血清HMGB1含量和外周血CD14+单核细胞表面TLR2、TLR4的表达率及蛋白相对含量明显高于非活动期和对照者(P<0.05);非活动期RA患者TLR4显著高于对照组(P<0.05)。活动期RA患者血清HMGB1与外周血CD14+单核细胞表面TLR2、TLR4相对蛋白含量表达呈显著正相关。结论 RA患者血清HMGB1及外周血单核细胞表面TLR2、TLR4的表达可能与RA病情发生发展有关,且具协同作用。Objective To prove the expression of HMGB1 and TLR2/TLR4 in the patients with rheumatoid arthri- tis. Methods To select 29 active RA, 20 inactive RA and 18 controls. The expression of HMGB1 in serum of these cases were detected by ELISA. The expression of TLR2 and TLR4 on CD14 ＋ monocytes in peripheral blood were detected by FCM. Results The HMGB1 content and the expression and relative protein quantification of TLR2 and TLR4 on CD14＋ monocytes in active RA was significantly higher than inactive group and controls; The expression of and relative protein quantification of TLR4 CD14 ＋ monocytes in inactive group is higher than con- trols. The serum level of HMGB1 in serum in active RA was positively correlated with the relative protein quantifi- cation of TLR2 and TLR4. Conclusions The expression of HMGB1 and TLR2/TLR4 in RA may be associated with the occurrence and development of HA and synchronous in rheumatoid arthritis.

关 键 词：类风湿关节炎 高迁移率族蛋白1 TOLL样受体 

分 类 号：R593.22[医药卫生—内科学]