马尾松菌根化苗木对干旱的生理响应及抗旱性评价  

Physiological responses of mycorrhizal Pinus massoniana seedlings to drought stress and drought resistance evaluation

作　　者：王艺[1,2] 丁贵杰[1] 

机构地区：[1]贵州大学造林生态研究所,贵阳550025 [2]贵州民族大学,贵阳550025

出　　处：《应用生态学报》2013年第3期639-645,共7页Chinese Journal of Applied Ecology

基　　金：国家"863"项目(2011AA100203);国家自然科学基金项目(31260183);国家科技支撑计划项目(2006BAD24B0301);贵州省重大科技专项(黔科合重大专项字[2012]6001号);贵州省特助人才计划(TZJF-2007年20号);贵州省人才基地建设项目(黔人领发[2009]9号);贵州省创新团队建设项目(黔科合人才团队[2011]4003);贵州大学研究生创新基金项目(省研农2009002)资助

摘　　要：采用温室盆栽方法,研究了持续干旱及复水处理后,接种褐环乳牛肝菌7、牛肝菌1、鸡油菌、彩色豆马勃和土生空团菌的马尾松苗木生理变化,并对菌根化苗木进行抗旱性评价.结果表明:在持续干旱条件下,马尾松苗木的丙二醛和相对质膜透性随之增加,但菌根化苗木的丙二醛和相对质膜透性均显著低于未接种苗木(对照);复水后,菌根化苗木中丙二醛和质膜透性较对照迅速降低.在持续干旱胁迫前21d,马尾松苗木超氧阴离子自由基产生速率增加,同时也诱导了菌根化苗木中过氧化物歧化酶、过氧化物酶和硝酸还原酶活性显著增加.随着胁迫时间的延长,苗木复水后的恢复能力各异.在胁迫14d复水后,苗木过氧化物歧化酶、过氧化物酶和硝酸还原酶的活性均得以恢复.菌根化苗木抗旱性的强弱为牛肝菌7>牛肝菌1>鸡油菌>土生空团菌>彩色豆马勃.过氧化物歧化酶和丙二醛与马尾松菌根化苗木抗旱性关联度较大,可以作为抗旱鉴定的主要指标.A greenhouse pot experiment was conducted to study the effects of inoculating Pisolithus tinctorius, Cenococcum geophilum, Cantharellus cibarius, and Suillus luteus on the physiological characteristics of Pinus massoniana seedlings under the conditions of drought stress and re-watering, with the drought resistance of the mycorrhizal seedlings evaluated. Under content and membrane＇ s relative permeability of P. massoniana seedlings drought stress, the MDA increased, but these two indices in the inoculated （mycorrhizal） seedlings were significantly lower than these in the un-inoc- ulated （control） seedlings .After re-watering, the MDA content and membrane＇ s relative permea- bility of mycorrhizal seedlings had a rapid decrease, as compared with the control. In the first 21 days of drought stress, the production rate of superoxide radical of the seedlings increased, and the SOD, POD and NR activities of mycorrhizal seedlings increased significantly. With the extending of drought stress, the seedlings after re-watering had different recovery ability. Under the re-watering after 14 days drought stress, the SOD, POD and NR activities recovered. The drought resistance of the mycorrhizal seedlings was in the order of Suillus luteus 1 〉 Suillus luteus 7 〉 Cantharellus cibari- us 〉 Cenococcum geophilum 〉 Pisolithus tinctorius. The SOD and MDA activities had a greater cor- relation with the mycorrhizal seedlings drought resistance, being able to be used as the indicators to evaluate the drought resistance of mycorrhizal seedlings.

关 键 词：马尾松 菌根化苗木 干旱 生理响应 抗旱性评价 

分 类 号：S791.248[农业科学—林木遗传育种]