来自中国北方部分地区33株孢子丝菌菌株的表型和基因型鉴定  

Reclassification of 33 clinical strains of Sporothrix from northern China based on phenotypic and molecular characters

作　　者：谭静文[1,2] 刘伟[1,2] 万喆[1,2] 李若瑜[1,2] 

机构地区：[1]北京大学第一医院皮肤性病科 [2]北京大学真菌和真菌病研究中心皮肤病分子诊断北京市重点实验室,北京100034

出　　处：《菌物学报》2013年第2期161-167,共7页Mycosystema

基　　金：教育部"新世纪优秀人才支持计划"(No.NCET-10-0198);国家自然科学基金重点项目(No.30930006);国家自然科学基金(No.30970131);北京市自然科学基金(No.7102149)

摘　　要：孢子丝菌病是一种临床常见的真菌感染疾病,近期有研究认为其致病菌是由多个同形种构成的复合体。为明确我国孢子丝菌病致病菌的生理学以及分子生物学方面的特点,对33株分离自我国孢子丝菌病患者组织的病原菌进行了研究,首先检测其在37℃是否生长以及PDA培养基上生长21d的菌落直径,其次检测其糖同化特点,最后对其钙调蛋白(Calmodulin,CAL)基因进行PCR扩增、序列测定以及系统进化分析。结果显示,这些菌株全部为球形孢子丝菌Sporothrix globosa,说明我国孢子丝菌病的病原菌可能以球形孢子丝菌为主。Sporotrichosis is a common fungal infection. Recent studies have demonstrated that the pathogen of this disease constitutes a complex of several species. For the purpose to study physiological and molecular characters of this fungal pathogen in China, 33 clinical strains of Sporothrix spp. were chosen in this study. First, we evaluated the growth ability at 37℃ and measured their colony diameters of these strains in 21 days after incubation on PDA. Second, we evaluated the ability to assimilate carbon resources. Last, we performed the phylogeny analysis based on the sequences of their calmodulin genes after being amplified and sequenced. The results demonstrated that these strains were all identified as Sporothrix globosa, indicating S. globosa may be the major cause of sporotrichosis in China.

关 键 词：孢子丝菌 生理学 分子生物学 

分 类 号：R519[医药卫生—内科学]