风湿性二尖瓣狭窄伴心房颤动患者血红素氧合酶-1的表达  

Expression of heme oxygenates-1 in atrial fibrillation patients with rheumatic mitral stenosis

作　　者：任贝贝[1] 郭俊杰[2] 王雪春[2] 李永红[2] 

机构地区：[1]青岛大学医学院,山东青岛266003 [2]青岛大学医学院附属医院心内科

出　　处：《临床心血管病杂志》2013年第3期176-178,共3页Journal of Clinical Cardiology

基　　金：青岛市科学技术局资助项目(No:02-2-kj-yn-25)

摘　　要：目的:观察血红素氧合酶(HO)-1与风湿性二尖瓣狭窄伴心房颤动(房颤)患者心肌重构的关系,探讨HO-1在房颤心肌重构中的作用及机制。方法:选取风湿性心瓣膜病行换瓣手术患者60例,将其分为窦性心律组(21例)及房颤组(39例),术前常规行心电图、超声心动图、X线等相关检查。外科换瓣手术时取右心耳组织约200mg,通过组织学检查、逆转录聚合酶链反应和Western blot技术分别检测心肌纤维化程度、HO-1及丝裂原活化蛋白激酶p38的表达水平。结果:①HE染色及Masson染色显示,房颤组心肌纤维化程度较窦性心律组显著;②与窦性心律组相比,房颤组HO-1mRNA及蛋白表达均明显上调,p38蛋白表达明显升高(均P<0.05)。结论:风湿性心脏病房颤患者心房重构过程中,HO-1和p38的表达均显著升高;HO-1表达增高可能与p38通路的激活有关。Objective：To study the relationship between HO 1 and fibrosis of atrial fibrillation （Af） in rheumatic mitral stenosis patients, and investigate the effect of HO 1 in fibrosis of Af. Method：Patients with rheumatic heart disease who needed valve replacements were divided into sinus rhythm group （n = 21 ） and Af group （n = 39）. Routine examinations, such as electrocardiogram, echocardiogram and X ray were taken before the operations. During the operation, 200 mg right atrial tissue was cut down to test the expression of HO- 1, the level of p38 and the process of intimal fibrosis by RT PCR technique, Western blot, HE stain and Masson stain, respectively. Result： （1）HE stain and Masson stain showed more myocardial fibrosis in Af group. （2）Compared with sinus rhythm group, the mRNA expression of HO-1 and p38 were obviously upregulated in Af group （P〈0.05）. Conclusion：The expression of HO-1 and p38 are both elevated in Af patients with rheumatic heart disease. The effect of HO-1 in Af may be related to the activation of p38 passageway.

关 键 词：心房颤动 血红素氧合酶-1 丝裂原活化蛋白激酶P38 

分 类 号：R541.7[医药卫生—心血管疾病]