电针对大鼠脊髓压迫性损伤后髓鞘再生的影响  

Effects of electro-acupuncture on remyelinization after compressive spinal cord injury

作　　者：黄思琴[1] 漆伟[2] 孙善全[2] 汪克建[2] 卓飞[2] 

机构地区：[1]重庆医科大学中医药学院,重庆400016 [2]重庆医科大学中医药学院解剖学教研室,重庆400016

出　　处：《中华物理医学与康复杂志》2013年第3期161-166,共6页Chinese Journal of Physical Medicine and Rehabilitation

基　　金：国家自然科学基金（30270437）;重庆市自然科学基金（cstc2012jjA10020）;重庆市卫生局中医药科技项目（2012-2-138）;重庆市教委科学技术研究项目（KJ120312）;重庆市渝中区科技计划项目（20110316）

摘　　要：目的观察电针对大鼠脊髓压迫性损伤（CSCI）后DNA结合抑制物2（Id2）和髓鞘碱性蛋白（MBP）的表达变化，探讨髓鞘再生的机制。方法将54只SD大鼠分为对照组和治疗组，每组27只，再根据取材时间点的不同各分为3d、7d和14d三个亚组，每个亚组9只大鼠。治疗组采用自行设计的大鼠脊髓压通器制作脊髓压通性损伤模型，针刺脊髓损伤节段局部夹脊穴、双侧足三里和双侧太溪穴，并于双侧足三里和太溪穴进行电针刺激（连续波，输出频率为2Hz，电压1．5V，30min）。对照组只造模，不治疗。2组大鼠均于对应时间点取材，运用电镜观察脊髓损伤节段髓鞘的超微结构；采用免疫印迹技术和免疫荧光双标从分子水平检测Id2及MBP的表达变化。结果CSCI后，荧光双标结果显示，造模后第3天，对照组大鼠的Id2免疫阳性少突胶质细胞数目为（20±2）个／高倍镜视野，并在造模后第14天下降至（164-1）个／高倍镜视野，组内各时间点Id2免疫阳性少突胶质细胞数目比较，差异无统计学意义（P〉0．05）。造模后第3天，治疗组大鼠Id2免疫阳性少突胶质细胞为（13±1）个／高倍镜视野，造模后第14天降至（7±1）个／高倍镜视野，组内各时间点ld2免疫阳性少突胶质细胞数目比较，差异有统计学意义（P〈0．05），并与同时间点的对照组比较，差异亦有统计学意义（P〈0．05）。Western结果显示，造模后第3天，对照组和治疗组Id2蛋白表达分别为（1．12±0．12）和（0．67±0．01），造模后第14天，对照组和治疗组Id2蛋白表达分别下降至（0．86±0．02）和（0．25±0．01），与组内造模后第3天比较，差异均有统计学意义（P〈0．05），2组相同时问点Id2蛋白表达组间比较，差异有统计学意义（P〈0．05）。造模后第3天，对照组和治疗组的MBP蛋白表达分别为（0．44±0．02）和（0．67±O．0Objective To observe the effects of electro-acupuncture on the expression and inhibition of DNA binding protein 2 （Id2） and myelin basic protein （MBP）, and to explore the mechanism of remyelinization after compressive spinal cord injury （CSCI） in rats. Methods Fifty-four SD rats were randomly divided into a control group and a treatment group, and each was further subdivided into 3 time point subgroups： 3, 7 and 14 days. There were 9 rats in each subgroup. The CSCI models were made with a self-designed method. The acupuncture points Jiaji （EX-B2）, bilateral Zusanli （ST36） and Taixi （KI3） were selected for treatment. Electro-acupuncture （ continuous wave, 2 Hz, 1.5 V） was applied to the bilateral Zusanli （ST36） and Taixi （ KI3 ） points. The control group received the injury but no treatment. The changes in the ultrastucture of the nerve fibers＇ white matter were determined by transmission electron microscopy （TEM）. The alterations in the expression of MBP and Id2 were observed by double labeled immunofluorescence and Western blotting on the 3rd, 7th and 14th day after the injury. Results TEM showed that the myelin sheaths in the control group had degenerated, swollen, and even broken down after CSCI. Changes to the myelin sheaths in the treatment group were milder than those in the control group. The immunofluorescence results showed the amount of Id2-immunoreactive oligodendrocytes in the control group to be （20 ± 2） on the 3rd day after CSCI, becoming （ 16± 1 ） on the 14th day. The differences among the 3 control subgroups were not statistically significant. The amount of Id2-immunoreactive oligodendrocytes in the treatment group was （13 ± 1 ） on the 3rd day, reaching a minimum the 14th day. The differences among the 3 treatment groups were statistically significant. The differences compared with the control group at the same time points were also statistically significant. Western blotting showed that the expression of Id2 in the contrast and treatment

关 键 词：脊髓压迫性损伤 脱髓鞘病变 电针 超微结构 髓鞘碱性蛋白 

分 类 号：R24[医药卫生—中医临床基础]