CD59在T细胞LAT脂筏内移位及跨膜信号转导中的作用  

The effect of CD59 on LAT in T cell signal transduction

作　　者：高美华[1] 秦云[1] 王冰[1] 张蓓[1] 张树超[1] 

机构地区：[1]青岛大学医学院免疫学教研室,266071

出　　处：《免疫学杂志》2013年第4期301-304,共4页Immunological Journal

基　　金：国家自然科学基金资助项目(81273206)

摘　　要：目的用前期构建好的LAT-EGFP质粒转染Jurkat细胞,探讨单克隆抗体交联CD59前后CD59与接头蛋白分子LAT在Jurkat细胞活化转导中的相关作用。方法采用电转染的方法转染Jurkat细胞,抗体交联CD59后共聚焦显微镜下观察细胞膜上LAT-EGFP分子分布的变化;利用噻唑蓝(MTT)比色法检测CD59抗体交联前后Jurkat细胞的增殖效应;用Western blot技术检测CD59抗体交联前后LAT磷酸化情况。结果共聚焦显微镜下可观察到CD59抗体交联后LAT-EGFP由散在分布为主变为点簇状分布为主;CD59抗体交联刺激组Jurkat细胞增殖能力增加;Western blot结果表明CD59抗体交联后LAT磷酸化水平增加。结论抗体交联CD59能使接头蛋白分子LAT进入脂筏,并增强T细胞信号转导效应。To observe how CD59 connected with LAT in T cell signal transduction, we transfected Jurkat cells with recombinant LAT-EGFP plasmid by electroporation. After cross linkage with anti-CD59 mAb, the Jurkat cells were monitored by confocal microscopy for the location of the fusion protein LAT-EGFP, and also measured by MTT colorimetry for the cell proliferation activity. Furthermore, Western blotting was used to test the phosphorylation levels of LAT. The results showed that, after the cross linkage with anti-CD59 mAb, LAT-EGFP clustered together, and the cell proliferation activity of Jurkat cells increased. The phosphorylation level of LAT elevated. Thus, we concluded that CD59 is related with LAT in T cell signal transduction and have synergistic effect on T cell signal transductinn

关 键 词：CD59 LAT JURKAT细胞 信号转导 

分 类 号：R392.11[医药卫生—免疫学]