马氏钳蝎短链神经毒素BmP03的溶液结构的NMR研究  

Three - Dimensional Structure of BmP03 from Venom of Scorpion Buthus martensii Karsch

作　　者：何发虎[1] 李医明[1] 吴宫[1] 曹春阳[1] 吴厚铭[1] 

机构地区：[1]中国科学院上海有机化学研究所,上海200032

出　　处：《化学学报》2000年第7期850-855,共6页Acta Chimica Sinica

基　　金：国家自然科学基金(29673055);国家科技部和中国科学院资助项目

摘　　要：BmPO3为从马氏钳蝎中得到的具有钾离子通道阻断活性的短链神经毒素.应用2D NMR实验和分子模拟技术,进行BmPO3的溶液结构计算,结果显示BmPO3,与从蝎毒中得到的其他短链神经毒素具有相似结构.含一个α-螺旋(Cys3-Gly12),两条反平行的β-折叠股(Asn16-Cys19,Cys24-Asn27).螺旋与折叠股间靠3对二硫键相连,在Asp20到Va123间形成一个二型转角结构.根据BmPO3的溶液结构,对其表面电荷对钾离子通道阻断活性的影响进行观察.From the venom of scorpion Buthus martensii Karsch, a short peptide ( BmP03, 28 amino acid residues) was isolated, characterized and tested as a weak inhibitor of K+ channel. In this paper, the solution structure of BmP03 was determined by 2D 1H NMR spectroscopy and molecular modeling calculations. The conformation of BmP03 is composed of a short α- helix (Cys3 - Gly12) and a two - strand antiparallel β-sheet (Asn16- Cys19,Cys24 - Asn27). There are three disulfide bridges (Cys3 - Cys19, Cys6 - Cys24, Cys10 - Cys26) connecting the a - helix and β- sheet. Asp20 to Val23 residues form a type Ⅱ turn linking the two strands. Structural and electrostatic potential comparison between BmP03 and its analogues were also presented.

关 键 词：马氏钳蝎 神经毒素 BmP03 钾离子通道 溶液结构 

分 类 号：O657.2[理学—分析化学]