罗格列酮对脓毒症大鼠高血糖与胰岛素抵抗的影响  

Impact of rosiglitazone on hyperglycemia and insulin resistance in septic rats

作　　者：郭鸿[1] 刘丽平[1] 张斌[1] 石斌[1] 李斌[1] 

机构地区：[1]兰州大学第一医院重症医学科,兰州甘肃730000

出　　处：《中国普通外科杂志》2013年第3期300-304,共5页China Journal of General Surgery

基　　金：教育部中央高校博士点基金资助项目(20090211120015);甘肃省卫生厅"陇原青年创新人才扶持计划"资助项目

摘　　要：目的:探讨罗格列酮对盲肠结扎穿刺(CLP)所致脓毒症大鼠高血糖及胰岛素抵抗的影响。方法:18只SD大鼠随机均分为假手术组,脓毒症组,脓毒症+罗格列酮预处理组(罗格列酮组)。大鼠脓毒症采用CLP方法诱导,罗格列酮组大鼠于CLP前30 min给予罗格列酮(20 mg/kg)灌胃。大鼠分别于术前及术后30,60,90,120,150 min经尾静脉采血检测空腹血糖(FPG);术后150 min处死大鼠,检测空腹血胰岛素水平(FINs),计算胰岛素抵抗指数(HOMA-IRI),检测血清与空肠组织TNF-α浓度。结果:脓毒症组大鼠FPG较术前明显升高,且术后各时间点FPG均明显高于假手术组(均P<0.05),而罗格列酮组大鼠FPG升高不明显,术后各时间点FPG与假手术组比较,差异均无统计学意义(均P>0.05);脓毒症组FINs,HOMA-IRI以及血清与空肠组织TNF-α浓度均明显高于假手术组(均P<0.05),罗格列酮组各项指标改变均不明显,与假手术组间差异均无统计学意义(均P>0.05)。结论:罗格列酮能改善GLP诱导的脓毒症大鼠的胰岛素抵抗,并降低全身炎症反应。Objective： To investigate the effects of rosiglitazone on cecal ligation and puncture （CLP） induced sepsis. hyperglycemia and insulin resistance （IR） in rats with Methods： Eighteen SD rats were equally randomized into sham operation group, sepsis group and rosiglitazone pretreatment plus sepsis group （rosiglitazone group）. Sepsis in rats was induced by means of CLP, and rats in rosiglitazone group were treated with rosiglitazone （20 mg/kg） by garage 30 min before CLP. The fasting plasma glucose （FPG） levels in rats were measured in tail-vein blood samples before and 30, 60, 90, 120and 150 min after CLP. The rats were sacritlced at 13U mm, the fasting insuun （FINs） levels were determined, and the homeostasis model assessment insulin resistance indexes （HOMA-IRI） were calculated. In addition, the TNF-α concentrations in rat serum and jejunal tissues were measured. Results： The FPG level in rats of sepsis group was significantly increased compared with the level before surgery3 and the FPG levels at each time point were all significantly higher than those of sham operation group （all P〈0.0S）, while the change in FPG level in rats of rosiglitazone group was not evident, and its FPG levels showed no statistical differences compared with sham operation group at each time point （all P〉0.05）. The FINs level and HOMA-IRI as well as the TNF-α concentrations in the serum and jejunal tissues in sepsis group were all significantly higher than those in sham operation group （all P〈0.05）, but these parameters in rosiglitazone group changed unremarkably and presented no statistical differences versus sham operation group （all P〉O.O5）. Conclusion： Rosiglitazone pretreatment can improve the IR state, and also reduce the systemic inflammatory response in rats with CLP induced sepsis.

关 键 词：脓毒症 高血糖症 胰岛素抗药性 罗格列酮 

分 类 号：R631[医药卫生—外科学]