吸头小柱微萃取-解吸电晕束电离质谱用于快速分析体液中的降压药物  

Coupling Micropipette Tip-based Micro-extraction with Desorption Corona Beam Ionization Mass Spectrometry for Rapid Analysis of Antihypertensive Drugs in Body Fluid

作　　者：游金清[1] 郝艳红[1] 黄云清[1] 程玉鹏 孙文剑 余琼卫[1] 袁必锋[1] 冯钰锜[1] 

机构地区：[1]生物医学分析化学教育部重点实验室武汉大学化学与分子科学学院,武汉430072 [2]岛津分析技术研发(上海)有限公司,上海201201

出　　处：《分析化学》2013年第3期319-324,共6页Chinese Journal of Analytical Chemistry

基　　金：国家自然科学基金项目(Nos.91017013;31070327;20921062;21005057)资助

摘　　要：将C18 ZipTip吸头小柱微萃取(Micropipette tip-based micro-extraction)与解吸电晕束电离质谱(Desorption corona beam ionization mass spectrometry,DCBI-MS)联用,用于快速分析复杂生物样品中的小分子药物。吸头小柱微萃取装置简单、操作快速,适合作为与常压直接离子化质谱技术联用的前处理技术,能实现分析物的富集和基质干扰的消除。优化了萃取条件和离子源的参数,最终选择20%(V/V)乙腈溶液作为上样溶液,4!L乙腈作为解吸液。分析全过程包括萃取、自然挥干、电晕束解析电离等步骤,并可以在3 min内完成。此方法用于血浆中的地平类降血压药及尿液中氯胺酮的快速分析,结果满意。这种方法拓展了常压直接离子化质谱技术在体液分析中的应用。We developed a convenient method by c0upling micropipette tip-based micro-extraction （TBME） with desorption corona beam ionization mass spectrometry （DCBI-MS） for rapid analysis of medicines in biological samples. The operation of TBME is easy and fast, and it can enrich the analyte concentration and eliminate background interference, which makes it suitable as a novel sample preparation method before ambient Ionization. We have systematically optimized the extraction conditions and the instrument parameters. The typical analysis procedure includes the steps of extraction, drying and DCBI-MS detection, and the all processes take less than three minutes. The method was successfully used for the analysis of antihypertensive drugs such as nifedipine, nitrendipine and nimodipine and illegal drugs in body fluid. The method is of considerable interest for broadening the application of ambient ionization mass spectrometry for body fluid analysis.

关 键 词：解吸附电晕束离子化 吸头小柱微萃取 体液分析 降血压药 氯胺酮 

分 类 号：R446.1[医药卫生—诊断学] O657.63[医药卫生—临床医学]