人脐带间充质干细胞治疗脊髓损伤及其对内源性细胞增殖的影响  

Human umbilical cord mesenchymal stem cells for spinal cord injury and the effects on the proliferation of endogenous cells

作　　者：黄华[1] 张文进[1] 关方霞[2] 田毅[1] 郭伟旭[1] 宗盛华[1] 徐丹[1] 胡祥[1] Eileen Shen 杨波[1] 

机构地区：[1]郑州大学第一附属医院神经外科,河南省高校临床医学重点学科开放实验室,郑州市450052 [2]郑州大学生物工程系,郑州市450052

出　　处：《实用医学杂志》2013年第6期880-882,共3页The Journal of Practical Medicine

基　　金：郑州大学研究生科研基金(编号:11Y13303);郑州大学211三期建设项目"干细胞基础与临床研究";国家自然科学基金(编号:81171177)

摘　　要：目的:探讨人脐带间充质干细胞(human umbilical cord mesenchymal stem cells,hUCMSCs)在脊髓损伤(SCI)后减轻神经功能缺损的疗效,其对内源性细胞增殖的潜在影响。方法:雄性成年SD大鼠46只,以改良Allen′s法制备脊髓T9节段挫伤模型,用评估后肢的运动功能,HE染色、免疫组化染色和图像分析法观察GFAP,Nestin的表达情况。结果:hUCMSCs组在移植后行为学评分(BBB)明显高于PBS组(P<0.05),且损伤腔隙比PBS组更小(P<0.05);移植组中BrdU阳性细胞比对照组增加显著。结论:hUCMSC促进内源性细胞的增殖,有助于脊髓损伤后功能恢复。Objective To investigate the efficacy of hman umbilical cord mesenchymal stein （：ells （hUCMSCs） in reducing neurological deficits alter spinal cord injury （SCI）, and to explore its potential impael on the proliferation of endogenous cells. Methods 60 male SD rats were randomly divided into control group （PBS group） and hUCMSCs transplantation group （hUCMSCs group）. The SC1 animal model was induced by new Allen＇s method. The motor function of hind limbs was evaluated by BBB scores. The expressions of （;FAP and Nestiu were measured by HE staining, immunohistochemieal staining, and imaging analysis. Results Alter transplantation, BBB scores of hUCMSCs gruup were significantly higher than those of PBS group （P 〈 0.05）, damage cavities were smaller than those of PBS group （P 〈 0.05）, and BrdU positive cells were increased significantly as compared to PBS group （P 〈 0.05）. Conclusions hUCMSC could promote the proliferation of endogenous cells, and contribute to the functional recovery after spinal cord injury.

关 键 词：脊髓损伤 间充质干细胞 移植 内源性细胞 

分 类 号：R651.2[医药卫生—外科学]