DDPH抑制HECCM促PASMC增殖时第二信使变化的研究  

Effects of Hypoxia and DDPH on the Change in Second Messengers during Proliferation of PASMC

作　　者：段秋红[1] 廖国宁[1] 雷景迈[1] 何善述[1] 

机构地区：[1]同济医科大学基础医学院生物化学教研室,武汉430030

出　　处：《同济医科大学学报》2000年第5期388-390,共3页Acta Universitatis Medicinae Tongji

基　　金：国家自然科学基金!资助 (No. 392 70 2 95 )

摘　　要：采用 MTT比色、荧光技术和放免技术观察了猪肺动脉低氧内皮细胞条件培养液 (HECCM)和 1- (2 ,6 -二甲基苯氧基 ) - 2 - (3,4-二甲氧基苯乙胺基 )丙烷盐酸盐 (DDPH)对猪肺动脉平滑肌细胞 (PASMCs)增殖、第二信使 ([Ca2 +]i 和 c AMP)的影响。结果显示 :1HECCM促进 PASMC增殖 ,而 DDPH可抑制之 ,抑制率为 77.46 %。 2HECCM升高 PASMCs内 [Ca2 +]i,而 DDPH可对抗此作用。 3HECCM组 PASMCs内 c AMP含量明显低于对照组(NECCM组 ) ,而 HECCM+DDPH组 PASMCs内 c AMP与By using cell culture, MTT colorimetry, fluorescence and bioluminnescence, and radioimmunoassay techniques, the effects of hypoxial endothelia cell conditioned medium (HECCM) and DDPH on the pulmonary artery smooth muscule cell (PASMC) proliferation and intracellular free calcium and cAMP concentrations were observed. The results showed that DDPH could inhibit PASMC proliferation induced by HECCM with the inhibition ratio being 77.46 %. DDPH could decrease the HECCM induced elevation of PASMC intracellular free calcium concentration and increase PASMC cAMP level. It was suggested that the mechanism of DDPH inhibiting PASMC proliferation may be contributed to preventing PKC signal pathway, but not excluding other pathway.

关 键 词：慢性低氧性肺动脉高压 丙烷盐酸盐 DDPH HECCM 

分 类 号：R543.2[医药卫生—心血管疾病] R972[医药卫生—内科学]