宝坻大蒜愈伤组织诱导及植株再生体系优化  

Callus Induction and Plant Regeneration System Optimization of the Baodi Garlic

作　　者：李转春[1] 王珍[1] 彭爱芳[1] 范宝莉[1] 刘晓颖[1] 王振英[1] 

机构地区：[1]天津师范大学生命科学学院,天津300387

出　　处：《天津农业科学》2013年第3期9-12,共4页Tianjin Agricultural Sciences

基　　金：科技部富民强县计划资助和国家自然科学基金资助(31071671)

摘　　要：以宝坻大蒜发芽叶为外植体,比较了不同培养基的诱导效率,优化了愈伤组织诱导及植株再生体系。试验结果表明,诱导愈伤组织的最佳培养基为MS+2,4-D2.0mg·L-1+NAA2.0mg·L-1+KT1.0mg·L-1;诱导不定芽的最佳培养基为MS+6-BA1.5mg·L-1+NAA1.0mg·L-1+KT1.5mg·L-1;诱导生根的最佳培养基为MS+NAA0.6mg·L-1+KT0.5mg·L-1。In this study, the sprout leaves of the Baodi garlic were used as explants, the best conditions for callus induction and plant regeneration were analyzed by comparing the induction effects of different media combinations. The results showed that the best medium for callus induction was MS＋2,g-D 2.0 mg·L-1＋NAA 2.0 mg·L-1 %KT 1.0 mg·L-1, the best medium for inducing adventitious buds was MS＋6-BA 1.5 mg·L-1＋NAA 1.0 mg·L-1＋KT 1.5 mg·L-1, and the best condition for root induction was MS＋NAA 0.6 mg·L-1KT 0.5 mg. L-~.

关 键 词：宝坻大蒜 发芽叶 愈伤组织 快繁 

分 类 号：Q945.41[生物学—植物学]