楂芪降糖汤对糖尿病肾病大鼠肾脏NF-κB及Desmin表达的影响  

Effect of Zhaqi Jiangtang Decoction on Expression of Renal NF-κB and Desmin in Diabetic Nephropathy Rats

作　　者：李晓萍[1] 李进[1] 黄学宽[1] 

机构地区：[1]重庆医科大学中医药学院,重庆401331

出　　处：《中药新药与临床药理》2013年第2期142-147,共6页Traditional Chinese Drug Research and Clinical Pharmacology

基　　金：重庆市卫生局科技项目(2009-B-144)

摘　　要：目的观察楂芪降糖汤对糖尿病肾病(DN)大鼠肾脏核转录因子(NF-κB),结蛋白(Desmin)表达的影响,探讨其部分作用机制。方法链脲佐菌素诱导建立DN大鼠模型,成模大鼠随机数字法分组。同时设正常对照组。各组按相应药物剂量灌胃。10周末,HE染色及透射电镜观察肾组织病理形态和超微结构改变;免疫组化观察肾组织NF-κB及Desmin的表达。结果光镜和电镜下模型组肾组织病理变化显著,且模型组肾组织NF-κB、Desmin表达均明显增加;楂芪降糖汤高剂量组和优降糖组表达较模型组显著下调,差异均有统计学意义(P<0.05)。结论楂芪降糖汤对DN大鼠肾损害有保护作用,其机制可能是通过降低NF-κB、Desmin表达实现的。Objective To investigate the effects of Zhaqi Jiangtang Decoction on the expression of renal NF-KB and Desmin in diabetic nephropathy （DN） rats. Methods The model rats of diabetic nephropathy were established by in- jecting streptozotocin （STZ）and then were randomly divided into different treatment groups by random number method. At the same time, normal control group was set up. After the establishment of the model, the relevant drugs were given intragastrically to different groups respectively. At the end of 10th week, the pathological changes of renal tissue were observed by HE staining under transmission electron microscope （TEM）. The expression levels of renal nuclear factor kappa B （NF-KB） and Desmin were determined by Immunohistochemical method. Results There were significant pathological changes of diabetic nephropathy under the light microscope and TEM in the model group, and the expres- sion levels of renal NF-KB and Desmin were significantly increased. The expression levels of renal NF-κB and Desmin were down-regulated in high-dose Zhaqi Jiangtang Decoction group and in glibenclamide group compared with the model group （P 〈 0.05）. Conclusion Zhaqi Jiangtang Decoction has protective effect on kidney damage in DN rats, and the mechanism may be achieved by reducing renal NF-KB and Desmin expression.

关 键 词：楂芪降糖汤 糖尿病肾病 核转录因子-ΚB 结蛋白 

分 类 号：R285.5[医药卫生—中药学]