益气除痰方对小鼠lewis肺癌细胞凋亡的影响  

Effect of Yiqi Chutan Decoction on Cell Apoptosis in Lewis Lung Cancer Mice

作　　者：方若鸣[1] 林丽珠[2] 杨文娟[1] 杨建猛[1] 

机构地区：[1]广州中医药大学,广东广州510405 [2]广州中医药大学第一附属医院,广东广州510405

出　　处：《中药新药与临床药理》2013年第2期147-151,共5页Traditional Chinese Drug Research and Clinical Pharmacology

基　　金：广东省人才计划项目(粤教函79号;20100913)

摘　　要：目的探讨益气除痰方对小鼠Lewis肺癌生长的抑制及诱导细胞凋亡的作用。方法复制C57BL/6小鼠Lewis肺癌模型,将96只接种Lewis肺癌的小鼠随机分为6组:益气除痰方高、低剂量组,顺铂对照组,顺铂联合中药高、低剂量组,模型组,每组16只。给药1周后停药观察1周,其中一半动物处死观察细胞凋亡相关指标,另一半动物用于观察生存期。结果与模型组比较,益气除痰方高、低剂量组均能明显降低瘤质量指数,差异有统计学意义(P<0.05,P<0.01)。益气除痰方低剂量组肺转移抑制率为28%、高剂量组为52%,顺铂对照组为50%,顺铂联合低剂量组为40%、高剂量组为50%,与模型组比较,差异均有统计学意义(P<0.05)。与模型组比较,益气除痰方组高、低剂量组和顺铂对照组、联合用药组均能明显提高肿瘤细胞凋亡率,差异均有统计学意义(P<0.05,P<0.01,P<0.001)。与模型组比较,益气除痰方低剂量组及顺铂对照组能有效延长模型小鼠的生存期,差异均有统计学意义(P<0.01)。结论益气除痰方有抑制肿瘤生长、延长肿瘤动物生存期及促进Lewis肺癌细胞凋亡的作用。Objective To study the effect of Yiqi Chutan Decoction （YCD） which has the action of strengthening qi and eliminating phlegm, on cell apoptosis in tumor tissue of C57BL/6 mice bearing Lewis lung cancer. Methods Ninety-six C57BL/6 mice bearing Lewis lung cancer were equally randomized into 6 groups, and they were high- and low-dose YCD groups, cisplatin group, high- and low-dose cisplatin and YCD groups, and negative control group. The remedy was administered orally for one week, and then the effect was evaluated by observing the apoptotic indexes in half of the mice , and by monitoring the survival time in the other half after withdrawal for one week. Results Com- pared with the negative control group, high- and low-dose YCD reduced the tumor mass index （P 〈 0.01 ）, and all of the medication groups inhibited the tumor metastasis to some degrees and decreased pulmonary metastasis nodule count. The inhibition rate for pulmonary metastasis was 28 % in low-dose YCD group, 52 % in high-dose YCD group, 50 % in cisplatin group, 40 % in low-dose cisplatin and YCD group , and 50 % in high-dose cisplatin and YCD group, the difference being significant（P 〈 0.05 ）. The medication groups had higher tumor cell apoptotic rate than the negative control group（P 〈 0.001 ）. Low-dose YCD and cisplatin prolonged the survival time of the model mice （P 〈 0.001 ）. Conclusion YCD can inhibit tumor growth, prolong the survival time of tumor mice, promote apoptosis in tumor tis- sue, and enhance the apoptosis-promoting effect of eisplatin.

关 键 词：益气除痰方 细胞凋亡 LEWIS肺癌 小鼠 

分 类 号：R285.5[医药卫生—中药学]