含碘中药复方对AIT大鼠甲状腺组织凋亡调控蛋白Fas、FasL、Bcl-2、Bax的影响  

Effect of Iodine-Containing Chinese Herbal Recipe on Apoptosis-regulation Protein Fas/FasL and Bcl-2/Bax in Thyroid Tissue of Autoimmune Thyroiditis Rats

作　　者：张兰[1] 邹晓宁[2] 姜维娜[1] 杨斌婕[2] 樊程程[2] 

机构地区：[1]辽宁中医药大学附属医院,辽宁沈阳110032 [2]辽宁中医药大学,辽宁沈阳110032

出　　处：《中药新药与临床药理》2013年第2期159-162,共4页Traditional Chinese Drug Research and Clinical Pharmacology

基　　金：辽宁省自然科学基金计划(20092038)

摘　　要：目的观察中药复方中不同剂量含碘中药(海藻、昆布)复方对实验性自身免疫性甲状腺炎(AIT)大鼠甲状腺自身抗体(TGAb、TPOAb)、凋亡调控蛋白Fas、FasL、Bcl-2、Bax的影响,并筛选中药复方中含碘中药的最佳剂量。方法取SD雌性大鼠75只,随机分为正常对照组,模型组,含碘中药复方低、中、高剂量组。酶联免疫法检测各组大鼠甲状腺抗体水平;免疫组化法检测各组大鼠甲状腺组织凋亡调控蛋白Fas、FasL和Bcl-2、Bax的变化。结果含碘中药复方各剂量组与模型组比较,甲状腺自身抗体水平均降低,甲状腺组织Fas、FasL、Bax的表达均减少,Bcl-2的表达增多,差异均有统计学意义(P<0.01);含碘中药复方中、高剂量组与低剂量组比较,差异有统计学意义(P<0.05),但中、高剂量组组间比较无明显差异(P>0.05)。结论含碘中药复方能够通过降低甲状腺自身抗体水平,抑制凋亡调控蛋白Fas、FasL、Bax的表达,增加Bcl-2的表达,起到治疗AIT的作用。Objective To observe the effects of compound herbs （Surgassum and Thallus Laminariae et Eckloniae） on Bcl-2, Bax of experimental autoimmune thyroiditis （AIT） recipe having different doses of iodine-containing Chinese autoantibody and apoptosis-regulated proteins Fas, FasL, rats for the exploration of its therapeutic mechanism, and to optimize the doses of iodine-containing Chinese herbs in the recipe. Methods Seventy-five conventional SD female rats were chosen for the experiment. After 15 rats were taken for the normal group, the left 60 were used for modeling. The model rats were divided into 4 groups, model control group, and low-, middle- and high-dose iodine-containing Chinese herbs groups. Enzyme-linked immunosorbent assay was applied to detect the levels of autoantibody, and im- munohistochemical method was used to observe the changes of apoptosis-regulated proteins Fas/FasL, Bcl-2/Bax in the thyroid tissue. Results The levels of autoantibodies were lowered, the expression levels of Fas/FasL and Bax were reduced, and the expression level of Bcl-2 was increased in iodine-containing Chinese herbs groups compared with the model control group （P 〈 0.01 ）. The above indexes in high- and middle-dose groups differed from the low-dose group （P 〈 0.05 ） but the indicators in high- and middle-dose groups showed no obvious difference（P 〉 0.05）. Conclusion Iodine-containing Chinese herbs can decrease the levels of autoantibody, inhibit the expression levels of apoptosis-reg- ulated proteins Fas, FasL and Bax, and increase Bcl-2 expression, which contributes to its therapeutic effect for AIT. The optimal dose of Surgassum and Thallus Laminariae et Eckloniae is 20 - 30 g.

关 键 词：含碘中药复方 自身免疫性甲状腺炎 FAS FASL Bcl-2 Bax 细胞凋亡 

分 类 号：R285.5[医药卫生—中药学]