复方姜黄素脂质立方液晶纳米粒中姜黄素和胡椒碱的包封率测定  

Entrapment Efficiency of Curcumin and Piperine in Compound Curcumin Lipid-based liquid Crystalline Nanoparticles

作　　者：付建武[1,2] 涂瑶生[1,2] 孙冬梅[2] 张建军[2] 施之琪[1] 

机构地区：[1]广州中医药大学,广东广州510405 [2]广东省中医研究所,广东广州510095

出　　处：《中药新药与临床药理》2013年第2期200-205,共6页Traditional Chinese Drug Research and Clinical Pharmacology

基　　金：广东省自然科学基金项目(S2011010005927);广州市科技计划项目(11C36080689)

摘　　要：目的建立复方姜黄素脂质立方液晶纳米粒姜黄素和胡椒碱包封率的测定方法。方法采用HPLC法测定，色谱柱为KromasilC18柱（4．6mm×250mm，4．6μm），流动相为乙腈-04％磷酸（48：52），流速为1mL·min-1，检测波长为343nm，430nm（波长转换时间：16．5min）；采用微柱离心法和透析法测定包封率。结果姜黄素在1．8081～18．081ng范围内线性良好（r=0．9998）；胡椒碱在3．3331～33．331ng范围内线性良好（r=0．9999）；微柱离心法和透析法测定姜黄素、胡椒碱的包封率分别为59．52％、56．02％和60．13％、55．86％。结论采用微柱离心法和HPLC法可以快速、准确地测定复方姜黄素脂质立方液晶纳米粒姜黄素和胡椒碱的包封率。Objective To establish a RP-HPLC method for the determination of the content and entrapment efficiency of curcumin and piperin in compound curcumin lipid-based liquid crystalline nanoparticles （LCNP）. Methods The contents of curcumin and piperin in compound curcumin lipid-based LCNP were determined by HPLC. The column was Kromasil C18（4.6 mm×250mm, 4.6μm）. The mobile phase was acetonitrile-0.4 % phosphoric acid（48 ： 52） and the flow rate was 1 mL·min-1. The detection wavelength was 343 nm for curcumin and 430 nm for piperin（the converted time set at 16.5 min）. The minicolumn centrifugation and dialysis methods were employed to isolate free curcumin and piperine for the entrapment efficiency. Results The curcumin was linear in the range of 1.8081-18.081 ng（r = 0.9998 ） and the piperine in the range of 3.3331 -33.331 ng （r =0.9999）. The entrapment efficiency of curcumin and piperine determined by minicolumn centrifugation was 59.52 % and 56.02 %, and was 60.13 % and 55.86 % by dialysis method, respectively. Conclusion Minicolumn centrifugation combined with HPLC is able to determine the entrapment efficiency of cureumin and piperin in compound curcumin lipid-based LCNP quickly, accurately and conveniently.

关 键 词：姜黄素 胡椒碱 脂质立方液晶 微柱离心 透析法 包封率 

分 类 号：R284.1[医药卫生—中药学]