‘解放钟’枇杷香树脂醇合成酶基因ejAS保守区的克隆  

Clone of Amyrin Synthase Gene Conservative District from Eriobotrya japonica ‘Jie Fang Zhong’

作　　者：刘小英[1,2] 苏明华[1,2] 吴少华[1] 李惠华[2] 

机构地区：[1]福建农林大学园艺学院,福建福州350002 [2]福建省亚热带植物研究所,福建省亚热带植物生理生化重点公共实验室,厦门361006

出　　处：《亚热带植物科学》2013年第1期1-4,共4页Subtropical Plant Science

基　　金：福建省自然科学基金(2012J05047);福建省公益类科研院所专项(2011R1012-1)

摘　　要：以‘解放钟’枇杷(Eriobotrya japonica‘Jie Fang Zhong’)叶片为材料,采用改良SDS法提取总RNA,通过同源克隆和巢氏PCR方法首次从枇杷叶片中分离得到AS基因的保守区序列。结果表明:该AS基因保守区长854 bp,编码284个氨基酸残基,命名为ejAS(GenBank注册号:JX173279.1);经比对,与同为蔷薇科的嘎啦果的同源性最高,达98%,与辽东楤木、乌拉尔甘草、绿玉树等均有70%以上的相似性。Taking the leaves of loquat as materials, the improved method SDS was used for the RNA isolation. Based on the extracted total RNA, the AS gene conservative district sequence was cloned by the method of homology cloning and nested PCR. The results demonstrated that the conserved sequence was 854 bp in length, coded 284 amino acids and was named ejAS （NCBI number： JX173279.1）. Though sequence analysis by Blast in NCBI, the results showed that the ejAS gene had the highest homology and similarity （98%） to the Malus x domestica, and shared above 70% homology with the reported Aralia elata, Glycyrrhiza uralensis, Polygala tenuifolia, etc.

关 键 词：枇杷 AS基因 基因克隆 同源性 

分 类 号：Q943.2[生物学—植物学]