氟化钠对成骨细胞增殖及分化影响的实验研究  

Experimental Study on the Influence of NaF on the Proliferation and Differentiation of Osteroblasts

作　　者：余达林[1] 崔舜[1] 

机构地区：[1]同济医科大学附属协和医院职业病科,武汉430022

出　　处：《同济医科大学学报》2000年第5期471-473,共3页Acta Universitatis Medicinae Tongji

摘　　要：观察不同剂量氟化钠 (Na F)对大鼠成骨细胞增殖分化的影响。成骨细胞经酶消化法从新生 2 4h Sprague- Dawley(SD)种乳鼠头盖骨分离得到。在 10 - 7m ol/ L～ 5× 10 - 4 mol/ L Na F中培养。用 MTT测定细胞增殖 ,并经生化法测定细胞碱性磷酸酶 (AL P)及放免法测定培养液中骨钙素含量反映细胞分化。结果表明 :Na F对成骨细胞增殖率的影响呈双向调节 ,表现为小剂量 Na F (10 - 7mol/ L、 10 - 6m ol/ L、 10 - 5 mol/ L )刺激细胞增殖 ,其提高增殖率分别达到 11.3%、10 .9%、 10 .6 % ,(P<0 .0 5 ) ,大剂量反之 ,与对照组相比 ,10 - 4 m ol/ L及 5× 10 - 4 mol/ L Na F降低细胞增殖率为 9.6 %与 9.7%。细胞分化对 Na F的反应表现为多样性。 AL P活性的改变基本与细胞增殖率的变化相同 ,即小剂量刺激 ,大剂量抑制。然而骨钙素分泌的改变则呈单向作用 ,各浓度 Na F均刺激细胞骨钙素分泌 ,其中 5× 10 - 4 mol/ L组骨钙素水平最高达 6 7.14% (P<0 .0 5 )。 Na F对大鼠颅骨成骨细胞增殖的影响呈双向调节 。The effects of different concentrations of NaF on the proliferation and differentiation of osteroblasts were observed. Osteroblasts were obtained from the calvaria of 24 h newborn Sprague Dawley (SD) rat by using enzymic degestion method and cultured in the medium containing 10 -7 mol/L to 10 -5 ×10 -4 mol/L NaF. The rate of cell proliferation, ALP activity and osterocalcin were examined by the methods of MTT, biochemical assay and radioimmunologic assay (RIA) respectively. The results showed that the effect of NaF on the proliferation of osteroblasts was regulated in a double phase manner: low doses of NaF (10 -7 mol/L, 10 -6 mol/L, 10 -5 mol/L) increased cell proliferation by 11.3 %, 10.9 % and 10.6 %( P <0.05)respectively, vice versa at high doses (10 -4 mol/L, 5×10 -4 mol/L). As compared with control group, high doses of NaF decreased the proliferation rate by 9.6 %, 9.7 % respectively. The response of cell differentiation to NaF was varied. The influence of NaF on ALP activity was similar to that of cell proliferation. However, all concentrations of NaF used stimulated the secretion of osterocalcin with the highest secreting rate being up to 67.14 % at 5×10 -4 mol/L NaF. It was concluded that the influence of NaF on the proliferation of rat calvarial osteroblasts regulated in a double phase manner, but the action on cell differentiation was varied.

关 键 词：氟化钠 成骨细胞 增殖 分化 实验研究 

分 类 号：R68[医药卫生—骨科学] R329.2[医药卫生—外科学]