Characterization of a newly Isolated Bacterium Pandoraea sp.B-6 capable of degrading kraft Lignin  

作　　者：郑玉 柴立元 杨志辉 张欢 陈跃辉 

机构地区：[1]Institute of Environmental Science & Engineering,School of Metallurgical Science and Engineering,Central South University [2]Chinese National Engineering Research Center for Control & Treatment of Heavy Metal Pollution

出　　处：《Journal of Central South University》2013年第3期757-763,共7页中南大学学报（英文版）

基　　金：Project(50925417) supported by the National Funds for Distinguished Young Scientists,China;Project(50830301) supported by the Key program of the National Natural Science Foundation of China;Project(2012BAC12B03) supported by the National 12th Five-year Plan for Science and Technology Support,China

摘　　要：A newly isolated bacterium was screened out for its survival on medium with 6 000 mg/L kraft lignin as the sole carbon source and energy, and identified as Pandoraea sp. by 16S rRNA gene sequence analysis. The biodegradation experiment was carried out in mineral salt medium, containing 5 000 mg/L kraft lignin as only carbon and energy at pH 8.0 and 30℃. Under these conditions, significant reduction in color and lignin content by the strain was observed after incubation for 5 d. The strain attains maximum reduction capability in color （44.6%） and lignin content （39.9%） within 5 d of incubation, and reduced chemical oxygen demand（COD） from initial concentration 7 399 to 3 980 mg/L at maximum reduction level of 46.2% on the 4th day. The total ion chromatograph （TIC） of compounds presented in the chloroform extract of control and bacterial treated samples shows the formation of several lignin-related aromatic compounds including some small molecular lignin fragments, indicating a strong destruction in the lignin structure.A newly isolated bacterium was screened out for its survival on medium with 6 000 mg/L kraft lignin as the sole carbon source and energy,and identified as Pandoraea sp.by 16S rRNA gene sequence analysis.The biodegradation experiment was carried out in mineral salt medium,containing 5 000 mg/L kraft lignin as only carbon and energy at pH 8.0 and 30 °C.Under these conditions,significant reduction in color and lignin content by the strain was observed after incubation for 5 d.The strain attains maximum reduction capability in color(44.6%) and lignin content(39.9%) within 5 d of incubation,and reduced chemical oxygen demand(COD) from initial concentration 7 399 to 3 980 mg/L at maximum reduction level of 46.2% on the 4th day.The total ion chromatograph(TIC) of compounds presented in the chloroform extract of control and bacterial treated samples shows the formation of several lignin-related aromatic compounds including some small molecular lignin fragments,indicating a strong destruction in the lignin structure.

关 键 词：kraft lignin Pandoraea sp. BIODEGRADATION gas chromatography-mass spectrophotometry relative molecular massdistribution 

分 类 号：X172[环境科学与工程—环境科学]