炎性痛大鼠背根神经节和脊髓背角Nogo—A蛋白表达的变化  

Changes in expression of Nogo-A protein in dorsal root ganglion and spinal dorsal horn in a rat model of inflammatory pain

作　　者：许丹阳[1] 陈雪梅[1] 唐莹[2] 张励才[3] 

机构地区：[1]内蒙古赤峰学院附属医院麻醉科,024000 [2]山东省潍坊市益都中心医院 [3]江苏省徐州医学院麻醉学重点实验室

出　　处：《中华麻醉学杂志》2013年第1期69-71,共3页Chinese Journal of Anesthesiology

摘　　要：目的评价炎性痛大鼠背根神经节和脊髓背角Nogo—A蛋白表达的变化。方法健康成年雄性SD大鼠120只，体重250—300g，采用随机数字表法，将其随机分为两组（n=60）：对照组（C组）和炎性痛组（F组）。F组大鼠左侧后肢趾部皮下注射3％福尔马林0．1ml，C组注射等容量的生理盐水。于注射后Ih、1、2、3和7d时采用vonFrey纤毛测定左侧后爪机械痛阈。于各时点随机取12只大鼠处死后取损伤侧LJ5背根神经节和L4，5脊髓背角，分别采用免疫荧光标记法和Westernblot法检测Nogo—A蛋白的表达。结果与C组比较，F组大鼠注射后1h、1、2、3和7d时机械痛阈降低，注射后1h、2、3和7d时背根神经节和脊髓背角Nogo-A蛋白表达上调（P〈0．05）。结论背根神经节及脊髓背角Nogo—A蛋白表达上调可能在福尔马林致大鼠炎性痛形成过程中发挥重要作用。Objective To evaluate the changes in the expression of Nngo-A protein in the dorsal root ganglion （DRG） and spinal dorsal horn in a rat model of inflammatory pain. Methods One hundred and twenty male Sprague-Dawley rats, weighing 250-300 g, were randomly divided into 2 groups （ n = 60 each） ： control group （group C） and formalin group （group F）. The inflammatory pain was induced by injection of 3% formalin 100 μl into the plantar surface of left hindpaw in group F. The equal volume of normal saline was given instead of formalin in group C. Mechanical withdrawal threshold to yon Frey filament stimulation was measured at 1 h and 1, 2, 3 and 7 days after injection. Twelve rats in each group were chosen at each time point and sacrificed. The L5 DRG and L4.5 segment of spinal cord on the operated side were removed for determination of Nogo-A protein expression by and Western blot. Results Compared with group C, the mechanical withdrawal threshold was significantly decreased at 1 h, 1, 2, 3 and 7 days after injection, and the expression of Nogo-A protein in the DRG and L4,5 segment of spinal cord was up-regulated at 1 h and 2, 3 and 7 days after injection in group F （ P 〈 0.05） .Conclusion Up-regulation of Nogo-A protein in the DRG and spinal dorsal horn may play an important role in the development of formalin-induced inflammatory pain in rats.

关 键 词：炎症 疼痛 脊髓 神经节 脊 Nogo—A蛋白 

分 类 号：R285.5[医药卫生—中药学]