cGMP依赖性蛋白激酶G抑制剂对内毒素孵育大鼠胸主动脉血管环反应性的影响  

Effect of cGMP-dependent protein kinase G inhibitor （D）-DT-2 on the lipopolysaccharide-induced hyporesponsiveness of rat vascular ring

作　　者：郭君[1] 王学敏[1] 王海燕[1] 薛瑛[1] 周全红[1] 江伟[1] 

机构地区：[1]上海交通大学医学院附属上海市第六人民医院麻醉科,200233

出　　处：《中华麻醉学杂志》2013年第1期109-112,共4页Chinese Journal of Anesthesiology

摘　　要：目的评价特异性cGMP依赖性蛋白激酶G（PKG）抑制剂（D）-DT-2对内毒素（LPS）孵育大鼠胸主动脉血管环收缩功能的影响。方法实验Ⅰ取SD大鼠胸主动脉血管环，采用随机数字表法分为3组（n=5）：KH液组、DL组和DDT2组，用50／μmol／L8-Br-cGMP孵育25min后加入上述各药，比较各组血管环的张力变化。实验Ⅱ将SD大鼠胸主动脉血管环，采用随机数字表法分为4组（n=5）：对照组、LPS组、LPS-DL组和LPS—DDT2组，在体外测定LPS孵育3h后各组血管环在苯肾上腺素（PE）作用下最大收缩力（Emax）以及产生最大收缩力的半数有效浓度（EC50）。结果实验IPKG抑制剂DT-2和（D）-DT-2能够使8-Br-cGMP舒张的血管环收缩，DDT2组血管环的Emax明显高于D｜r2组（P〈0．05）。实验Ⅱ DT-2和（D）-DT-2均可以显著提高LPS孵育血管环的收缩功能；与LPS组比较，LPS—DT2组和LPS—DDT2组Emax升高，EC50降低（P〈0．01）。LPS-DDT2组与LPS—DT2组相比Emax升高，EC50降低（P〈0．05）。结论PKG抑制剂可提高内毒素孵育血管环的收缩功能，（D）-DT-2恢复血管反应性的作用强于DT-2。Objective To investigate the effect of specific cGMP-dependent protein kinase G （PKG） inhibitor （D）-DT-2 on the contractile function of rat vascular rings after being exposed to lipopolysaccharide （LPS）. Methods The experiment was performed in 2 parts. Part 1 ： The Sprague-Dawley rat thoracic aortic rings were randomly divided into 3 groups （ n = 5 each） ： KH group, DT-2 group and （D）-DT-2 group. KH, DT-2 and （D）- DT-2 were added to the aortic ring after being dilated with 8-Br-cGMP 50 μmol/L for 25 min and the changes in tension of vascular rings were measured. Part H ： The rat thoracic aortic rings were randomly divided into 4 groups （n = 5 each）： control group, LPS group, LPS-DTz group and LPS-（D）-DT2 group. After being incubated with LPS for 3 h in vitro. The Emax and ECs0 were compared among the 4 groups. Results Part I ： Both DT-2 and （D）-DT-2 could contract the vascular rings dilated with 8-Br-cGMP and the Emax was significantly higher in （D）- DT-2 group than DT-2 group （P 〈 0.05）. Part II ： Both DT-2 and （D）-DT-2 significantly improved the contractile function of vascular ring after being exposed to LPS. Emax was significantly higher, while ECso was lower in groups DT-2 and （D）-DT-2 than in LPS group （P 〈 0.01）. Emax was significandy increased, while EC50 was decreased in LPS-（D）-DT-2 group as compared with LPS-DT-2 group （ P 〈 0.05）. Conclusion PKG inhibitor can improve the contractile function of the vascular rings incubated with LPS and the efficacy of （D）-DT-2 is better than DT-2 in recovering the vascular reactivity.

关 键 词：cGMP依赖性蛋白激酶G抑制剂 内毒素 胸主动脉血管环 

分 类 号：R285.5[医药卫生—中药学]