Fucoidan对Lewis肺癌荷瘤小鼠抑瘤及免疫调节作用  

Antitumor activity and Immunomodulation of fucoidan from Undaria pinnatifida on Lewis lung cancer-bearing mice

作　　者：李巧梅[1] 杨丽丽[1] 王培胜[1] 刘志超[1] 滕洪明[1] 邰宗洋[1] 邹向阳[1] 

机构地区：[1]大连医科大学生物技术系,辽宁大连116044

出　　处：《中国海洋药物》2013年第2期12-16,共5页Chinese Journal of Marine Drugs

基　　金：辽宁省教育厅基金项目(2009A199);辽宁省科技厅计划项目(2011225013)资助

摘　　要：目的研究褐藻多糖硫酸酯(Fucoidan)对Lewis肺癌小鼠抗肿瘤及免疫调节作用。方法建立Lewis肺癌荷瘤小鼠模型,腹腔注射给予不同浓度Fucoidan连续21d,检测Fucoidan对肿瘤生长、免疫器官指数。采用ELISA法检测荷瘤小鼠细胞因子水平。以CD34血管内皮细胞标记物,采用免疫组化法分析肿瘤内微血管生成情况。结果100和200mg.kg-1 Fucoidan显著抑制Lewis肺癌的生长,抑瘤率分别是42.54%,58.63%(P<0.05);显著增加荷瘤小鼠脾指数(P<0.05);50和100mg.kg-1组可提高荷瘤小鼠血清中IL-1β水平,100mg.kg-1可提高IL-2、IL-6和FAS-L水平;200mg.kg-1组血清IFN-γ水平显著提高(P<0.05);100和200mg.kg-1组肿瘤组织中微血管密度显著降低(P<0.05)。结论Fucoidan对小鼠Lewis肺癌的生长有抑制作用,可能与增强荷瘤小鼠抗肿瘤免疫、抑制肿瘤血管新生有关。Objective To investigate the antitumor activity and immunomodulation of fucoidan from Un- daria pinnatifida on Lewis lung tumor-bearing mice. Method Inhibitory rate and immune organs index of the fucoidan were detected in Lewis tumor-bearing mice in vivo. The level of cytokine secretion was measured by ELISA method. Tumor microvessel density （MVD） was observed by immunohistochemis- try method using CD34 as marker. Result fucoidan could significantly inhibit Lewis lung tumor growth, and the inhibition rate was 42. 54%, or 58. 63% at dose of 100 mg· kg-1 or 200 mg·kg-1 respectively for 21 days, being statistically significant compared with the control group （P〈0. 05）. The thymus and spleen index in experimental groups was significantly higher than that in control groups （P〈0. 05）. The levels of IL-1β, IL-2, IL-6, IFN-7, FAS-L in tumor-bearing mice serum, compared with control group, were significantly improved by fucoidan （P〈0. 05）. Tumor MVD was significantly decreased by fucoidan. Conclusion Fucoidan extracted from Undaria pinnatifida showed activities to kill tumor cells, promote immune responses and decrease MVD, suggesting its antitumor effects.

关 键 词：褐藻多糖硫酸酯 抗肿瘤 免疫调节 LEWIS肺癌 

分 类 号：R965[医药卫生—药理学]