多指标正交试验优化菊花提取工艺  

Optimization of extraction process of Chrysanthemi Flos by multi-index orthogonal experiment

作　　者：张星海[1] 李嬛[1,2] 秦昆明[1,2,3] 刘晓[1] 汪斌 蔡宝昌[1,2,3] 

机构地区：[1]南京中医药大学国家教育部中药炮制规范化及标准化工程研究中心,江苏南京210029 [2]南京海昌中药集团有限公司,江苏南京210061 [3]国家中医药管理局中药炮制标准重点研究室,江苏南京210046

出　　处：《中国中药杂志》2013年第6期821-824,共4页China Journal of Chinese Materia Medica

基　　金：国家科技部火炬计划项目(2010GH020663);南京市科技发展计划项目(201007005);江苏省企业博士集聚计划项目

摘　　要：目的:通过正交试验设计,探索量化的菊花评价指标,对菊花的提取工艺进行优化。方法:选择乙醇浓度、乙醇倍数、提取时间、提取次数4个因素,采用L9(34)安排试验,以菊花指纹图谱中8个主要共有峰的峰面积和浸出物含量作为指标,进行综合评价。结果:优选出菊花最佳提取工艺,采用超声波提取法,加入30倍80%乙醇溶液,提取40 min,共2次。结论:优化的提取工艺结果可靠,评价指标可控,对于规范菊花的提取工艺和菊花饮片的质量控制具有一定的意义。Objective ： To explore quantitative assessment indicators of Chrysanthemi Flos, and optimize the extraction process of Chrysanthemi Flos through orthognnal experimental design. Method： The concentration of ethanol, amount of ethanol, extraction time and extraction frequency were selected as factors in the L9 （34） orthogonal experiment. A comprehensive assessment was conducted with the peak area of the eight major common peaks in the fingerprint of Chrysanthemi Flos as the indicators. Result： The optimum extraction process was selected as follows ： using ultrasonic extraction method, adding 30-fold ethanol with 80% concentration, extracting for 2 times for extraction, 40 min for each time. Conclusion： The optimized extraction process is reliable, with controllable assessment indicators, which is significant to the standardization of the extraction process and quality control of Chrysanthemi Flos preparations.

关 键 词：菊花 正交试验 指纹图谱 多指标 提取工艺 

分 类 号：TQ461[化学工程—制药化工]