HIV感染者唾液人类疱疹病毒感染与CD4淋巴细胞的关系  

Relationship between incidence of multiple human herpesviruses in saliva and CD4 lymphocyte count from HIV type-infected persons

作　　者：李承文[1,2] 史会萍[3,4] 祁燕伟 李榕[3,4] 夏志刚[3,4] 白劲松 段开文[3,4] 

机构地区：[1]昆明医科大学第三附属医院 [2]云南省肿瘤医院科研科,云南昆明650118 [3]昆明医科大学附属延安医院 [4]昆明市延安医院口腔科 [5]昆明市第三人民医院感染一科

出　　处：《现代预防医学》2013年第6期1086-1088,共3页Modern Preventive Medicine

基　　金：国家自然科学基金资助项目(30860315);云南省应用基础研究项目(2008ZC146M)

摘　　要：目的探讨HIV感染者唾液常见人类疱疹病毒感染情况及其与CD4淋巴细胞计数的关系。方法利用PCR方法对245例HIV感染者唾液疱疹病毒1～4型即HSV-1、HSV-2、VZV和EBVDNA的存在情况进行检测,并分析检出率与CD4细胞的关系。结果 HIV感染者唾液HSV-1、HSV-2、VZV和EBV检出率分别为29%、3.3%、4.1%和82%。CD4淋巴细胞计数小于200个/μl时患者的唾液中更容易检测到HSV-1、VZV和EBV。CD4淋巴细胞计数与HSV-1和EBV同时存在明显相关(P=0.0001)。CD4淋巴细胞计数低于200个/μl的患者比CD4淋巴细胞计数大于400个/μl的患者唾液中同时检测到HSV-1和EBV的几率高,即CD4淋巴细胞计数越低,多种疱疹病毒同时检出几率越高。结论 HIV感染者唾液中人类疱疹病毒1型和4型检出率高,与免疫指标CD4有关。OBJECTIVE The aim of this study was to determine the incidence of HSV-1, HSV-2, VZV and EBV in the sali- va of human immunodefieiency virus type （HIV） -infected persons, and their correlation with CD4 lymphocyte count. METH- ODS Polymerase chain reaction （PCR） or nested PCR was used to investigate the presence of salivary HSV-1, HSV-2, VZV and EBV from 245 HIV-seropositive individuals from The Third Affiliated Hospital, Kunming Medical University. CD4 lympho- cyte count were also collected. Relationship between incidences of these human herpesviruses and CD4 count was analyzed. RE- SULTS In the HIV-seropositive person, the most prevalent viral DNA was EBV 82.0% （201/245） , followed by HSV-1 29.0% （71/245）, VZV 4.1% （10/245）, and HSV-2 3.3% （8/245）. When CD4 lymphocyte count less than 200/0,1, the saliva of patients more easily detected HSV-1, VZV and EBV. CD4 lymphocyte count and HSV-1 and EBV presence had sig- nificantly negative correlated （P = 0.000 1）. The detection rate of HSV-1 and EBV in saliva in the group of CD4 lymphocyte count below 200μl simultaneously was higher than CD4 lymphocyte count over 400/μl. CD4 lymphocyte count much lower, HHVs detection rate much higher, a negative correlation between CD4 lymphatic and detection rate of HHVs. CONCLUSION The observations reported herein suggest that the presence of the four HHVs determined by the PCR technique in saliva is a fre- quent event in HIV-infeeted individuals.

关 键 词：人类免疫缺陷病毒 唾液 CD4淋巴细胞 人类疱疹病毒 聚合酶链反应 

分 类 号：R512.91[医药卫生—内科学]