DIGFA-kit和Dot-ELISA检测斯氏狸殖吸虫抗体的研究  

Study on the detection of anti-pagumoganimus skrjabini antibody with DIGFA-kit and Dot-ELISA

作　　者：朱敬[1] 郑东[2] 朱名胜[3] 卫荣华[1] 杨树国[3] 

机构地区：[1]湖北医药学院生物学教研室,湖北十堰442000 [2]湖北医药学院口腔学院 [3]湖北医药学院寄生虫学教研室

出　　处：《现代预防医学》2013年第6期1092-1093,共2页Modern Preventive Medicine

基　　金：日本健康科学基金资助项目(JHSF;HS189)

摘　　要：目的探索用肺吸虫病金标渗滤试剂盒(DIGFA-kit)和斑点酶联免疫吸附试验(Dot-ELISA)检测斯氏狸殖吸虫抗体的敏感性和特异性。方法采用DIGFA-kit和Dot-ELISA检测流行区人群和病鼠血清抗体。结果斯氏狸殖吸虫病流行区人群和病鼠血清特异性抗体两种方法阳性率为5.1%、5.0%和100%、97.5%。用此两种血清学方法检测正常大鼠、旋毛虫病大鼠、血吸虫病兔和蛔虫病人血清,除1例血吸虫病兔血清DIGFA-kit出现阳性反应外,其他血清两种方法均为阴性反应。实验动物从感染后第2周两种方法开始抗体检测阳性,第4周阳性率均达90%以上,持续至8周。结论两种方法对斯氏狸殖吸虫特异性IgG抗体的检测均有较好的敏感性和特异性。OBJECTIVE To explore the sensitivity and specificity of detecting the anti-pagumoganimus skrjabini antibody by dot immunogold filtration assay （DIGFA） and dot enzyme-linked immunosorbent assay （Dot-ELISA）. METHODS The DIGFA and Dot-ELISA were used to test the anti-P.skrjabini antibody in sera from the crowd in poular area and infected rats. RE- SULTS The positive rats of the antibody in sera from crowd in popular area and infected rats all were 5.1%, 5.0 and 100%, 97.5% respectively. Compared with the results of healthy big rats, big rats with trichinosis, rabbits with schistosomiasis and as- cariasis cases, only one was positive with DIGFA in the rabbit of schistosomiasis. All of others in control group were negative. The antibody was detected at 2 weeks post-infectious in experimental animal. After 4 weeks, the positive rate reached 90% and lasted for 8 weeks. CONCLUSION The results show that these two methods have good sensitivity and specificity in detection of Pagumogonimus skrjabini antibodies.

关 键 词：斯氏狸殖吸虫 抗体 血清学试验 

分 类 号：R395.6[哲学宗教—心理学]