细胞色素P4502D6基因拷贝数多态性与血铅尿铅水平的关系研究  

The relationship between copy number polymorphisms of CYP2D6 gene and lead level of blood and urine in workers exposed to lead

作　　者：蒋琴琴[1] 刘斌[2] 刘移民[2] 

机构地区：[1]广州市疾病预防控制中心环境卫生科,广东广州510440 [2]广州市第十二人民医院(广州市职业病防治院)

出　　处：《现代预防医学》2013年第6期1097-1099,1104,共4页Modern Preventive Medicine

基　　金：广州市医药卫生科技重点专科项目(2007-Zdi-01);广东省科技计划项目(2008B060600008)

摘　　要：目的探讨铅作业人群细胞色素氧化酶P450酶系中的CYP2D6基因拷贝数多态性与铅毒性引起的血铅、尿铅水平的关系。方法选择广州市某蓄电池行业233名铅作业工人,按铅尘接触水平分为高浓度组和低浓度组2组,采用统一调查表进行职业性健康问卷调查;采用石墨炉原子吸收光谱法测定现场空气铅尘及铅作业工人血铅、尿铅水平;提取铅作业人群周围血DNA,采用实时荧光定量聚合酶链式反应(PCR)法和ΔCt值法检测铅作业工人的CYP2D6基因拷贝数,分析不同铅尘作业环境下作业人员CYP2D6基因拷贝数多态性与血铅、尿铅水平的关系。结果铅作业工人CYP2D6基因拷贝数与血铅、尿铅水平有关,2组中拷贝数小于2的作业人员血铅浓度均高于拷贝数为2的人群(P﹤0.001),低浓度组拷贝数小于2的研究对象尿铅浓度比拷贝数为2的人群高(P=0.03),尚未发现年龄、性别、工龄、学历与血铅水平和尿铅水平的关系差异有统计学意义。结论 CYP2D6基因拷贝数多态性与铅毒性引起的血铅尿铅水平有关,拷贝数少于2的CYP2D6基因可能是铅中毒易感基因型。OBJECTIVE To probe into the the relationship between blood lead and urine lead level and copy number polymor- phisms of CYP2D6 gene in workers exposed to lead. METHODS 233 cases of workers exposed to lead were scheduled for an in- terview to collect the subjects＇ data on occupational information. Their blood lead, urine lead and the ambient air lead were tested with graphite furnace atomic absorption spectrometry. CYP2D6 gene copy number of these workers was detected with real- time fluorescence quantitative PCR method and ACt method. Under different concentrations of environmental lead dust, analyze the relationship between CYP2D6 gene copy number polymorphisms and blood lead, urine lead level. RESULTS CYP2D6 gene copy number polymorphisms was relative with blood lead and urine lead level in these workers exposed to lead. In both higher and lower concentration of lead dust groups, the blood lead level in the subjects carried less than 2 copies of CYP2D6 gene were higher than those carried 2 copies （P 〈 0.001）. In lower concentration of lead dust group, the subjects carried less than 2 copies of CYP2D6 gene had higher urine lead level than the subjects with 2 copies （P = 0.03）. CONCLUSION CYP2D6 gene copy number polymorphisms is associated with blood lead and urine lead levelof lead toxicity, the CYP2D6 genotypes of less than 2 copy number may be susceptible to lead poisoning.

关 键 词：CYP2D6 拷贝数多态性 血铅 尿铅 

分 类 号：R598.11[医药卫生—内科学]