ATP生物荧光法快速监测外科手消毒的应用研究  

Application research on ATP bioluminescence assay in the evaluation of the effectiveness for monitoring surgical hand antisepsis rapidly

作　　者：柯雅娟[1] 许晨耘[1] 俞诗娃[1] 周淑萍[1] 冯菲菲[1] 

机构地区：[1]海南省人民医院手术室,海南海口570311

出　　处：《现代预防医学》2013年第6期1157-1158,共2页Modern Preventive Medicine

基　　金：海南省卫生厅立项课题(琼卫2011-29)

摘　　要：目的探讨三磷酸腺苷(ATP)生物荧光法在外科手消毒效果评价中应用的可行性及能反映外科手消毒合格的ATP相对吸光值。方法采用ATP生物荧光法与细菌培养计数法观察外科手消毒监测效果,用百分位数法计算ATP生物荧光仪的测量RLU值95%正常值上限,并进行成本比较。结果 ATP生物荧光仪测量的RLU值与细菌培养监测结果不成线性关系,RLU值95%正常值上限为48.02;其监测成本比细菌培养法节约59.89元。结论 ATP生物荧光法可快速监测外科手消毒效果,且操作成本较低,利于推广至现场快速监测工作。OBJECTIVE To discuss the application feasibility of the ATP bioluminescence assay in the evaluation of the effec- tiveness of surgical hand antisepsis and ATP relative extinction value which can reflect the disinfection qualified. METHODS We used the ATP bioluminescenee assay and bacilli culture notation respectively, to monitor the effectiveness of surgical hand antisepsis and calculate by method of percentiles to measure RLU value 95% normal value upper limit of ATP bioluminescence assay, then compared the cost. RESULTS The RLU value of ATP bioluminescence assay was not linear related to the results of bacilli culture, RLU value 95% normal value upper limit of ATP bioluminescence assay was 48.02, and the cost was 59.89 RMB less than bacilli culture. CONCLUSION ATP bioluminescence assay may appraise the effectiveness of monitoring surgical hand antisepsis rapidly and is lower cost, which can promote application on scene monitoring.

关 键 词：三磷酸腺苷生物荧光法 外科手消毒 监测 效果 

分 类 号：R613[医药卫生—外科学]