IL-4受体阻断对小鼠急性感染肝片吸虫后肝脏单核细胞悬液中细胞因子的影响  

Effects of Blockage of IL-4Rα on the Early Cytokine Profiles in Hepatic Mononuclear Cells from Mice Infected with Fasciola hepatica

作　　者：罗洪林[1] 王豪举[1] 张文韬[1] 郭智莉[1] 周雪梅[1] 周容琼[2] 周作勇[2] 

机构地区：[1]西南大学动物科技学院预防兽医学重庆市重点建设实验室,重庆400716 [2]西南大学荣昌校区动物医学系,重庆402460

出　　处：《中国畜牧兽医》2013年第3期118-122,共5页China Animal Husbandry & Veterinary Medicine

基　　金：中央高校基本科研业务费专项资金(XDJK2009B001)

摘　　要：本研究旨在探索肝片吸虫感染小鼠急性期肝脏单核细胞悬液中细胞因子表达模式及其原因。试验将小鼠分为4组,分别对感染抗体处置组、感染非抗体处置组、抗体处置非感染组和非抗体处置非感染组的肝脏单核细胞培养上清液中IL-4、IL-5、IL-13及IFN-γ进行测定;同时也对肝脏中IL-4及IL-12P40mRNA水平进行荧光定量PCR检测。结果显示,IL-4和IL-5的表达模式相似。在非感染状态下,无论是否进行抗体处置,两者都处于较低表达水平。在感染状态下,两者在非抗体处置小鼠中的表达显著高于抗体处置组(P<0.05)。IL-13与IL-4及IL-5的表达差异在于感染并用抗体处置后,小鼠肝脏单核细胞中的IL-13仍有较高水平表达。IFN-γ的表达量在感染后有所增加,但在有无抗体处理间无显著差异(P>0.05)。mRNA检测结果显示,在非感染状态下,抗体处置后的IL-4mRNA水平显著低于非抗体处置组(P<0.05)。感染后非抗体处置组小鼠中IL-4mRNA水平极显著高于抗体处置组(P<0.01),同时也极显著高于非感染非抗体处置组(P<0.01)。IL-12P40mRNA的水平在感染状态下,抗体处置组的IL-12P40mRNA虽较非抗体处置组低,但无显著差异(P>0.05)。但感染后非抗体处置组的IL-12P40mRNA水平均极显著高于感染组抗体处置和非抗体处置组(P<0.01)。本试验结果表明,IL-4Rα对肝片吸虫感染急性期IL-5和IL-4的表达极其重要,但对IL-13及IFN-γ的影响较小。The assay was aimed to explore the early cytokine profiles in hepatic mononuclear cell from mice infected with Fasciola hepatica.Mice were classed into 2 groups,infected and non-infected.In each group,mice were treated or untreated with IL-4R α antibodies,respectively.Hepatic mononuclear cells were harvested and cultured.The supernatant was used to measure the cytokine levels and the livers were used to measure mRNA of IL-4 and IL-12P40 by Real-time PCR.Results demonstrated that the profiles of IL-4 and IL-5 were similar.Both were quite low without infection of F.hepatica whatever the antibodies were treated or not.However,when mice were infected,both cytokines in mice without antibody were significantly higher than mice treated with antibody（P＆lt;0.05）.The IL-13 showed different profile to IL-4 with the high level in mice treated with antibody.IFN-γ increased when mice were infected,but no differences were found when mice were treated or untreated with antibody（P＆gt;0.05）.The mRNA level of IL-4 in mice treated with antibody but uninfected with F.hepatica was significantly lower than wild type mice（P＆lt;0.05）.However,it was extremly significantly higher in mice infected but untreated with antibody than mice that infected and treated with antibody（P＆lt;0.01）.mRNA level of IL-12P40 showed no differences between groups when mice were infected with F.hepatica.Our data showed that IL-4Rα was crucial to the expressions of IL-4 and IL-5,but not to the IL-13 and IFN-γ during the acute phase of F.hepatica infection.

关 键 词：肝片吸虫 IL-4受体 肝脏单核细胞 细胞因子 

分 类 号：S852.33[农业科学—基础兽医学]