雌激素对奶牛输卵管上皮细胞前列腺素E_2和F_(2α)合成酶mRNA表达的影响  

Effect of Estrogens on the Expression of PGES and PGFS mRNA in Bovine Oviduactal Epithelial Cells

作　　者：樊凤娇[1,2] 董至恒[1,2] 付改玲[1,2] 黄娜[1,2] 曹金山[1,2] 

机构地区：[1]内蒙古农业大学兽医学院,内蒙古呼和浩特010018 [2]农业部动物疾病临床诊疗技术重点实验室,内蒙古呼和浩特010018

出　　处：《中国畜牧兽医》2013年第3期130-133,共4页China Animal Husbandry & Veterinary Medicine

基　　金：教育部留学回国人员科研启动基金(201-204015);内蒙古自治区高等学校科学研究重点项目(NJ10049);国家自然科学基金(31160490)

摘　　要：为了揭示雌激素(estrogen,E2)对奶牛输卵管上皮细胞中前列腺素E2合成酶(PGES)和前列腺素F2α合成酶(PGFS)mRNA表达的影响,探讨E2对奶牛输卵管生殖生理的调节作用,本试验采用了体外培养荷斯坦奶牛输卵管上皮细胞技术,分不同时间(0、2、4、8、16、24和48h)和不同浓度(10-12、10-11、10-10、10-9 mol/L)添加雌激素E2(以不加雌激素作空白对照),采用荧光定量PCR技术检测PGES和PGFS mRNA表达。不同浓度的E2或同一浓度不同刺激时间的E2均能增加PGES的表达,但4h浓度为10-10 mol/L时与空白对照相比差异极显著(P<0.01),而PGFS在24h添加浓度为10-12 mol/L E2时与空白对照相比差异极显著(P<0.01)。试验结果表明,E2对培养的奶牛输卵管上皮细胞PGES和PGFS mRNA的表达有促进作用,说明雌激素E2对前列腺素酶PGES和PGFS mRNA的表达具有调控作用。In order to explore the expression of prostaglandin E2 and F2α synthase（PGES and PGFS） mRNA in bovine oviductal epithelial cells by estrogen,investigation the function of physiology in bovine.We used bovine regenerating epithelial cells in vitro training system,different times（0,2,4,8,16,24 and 48 h）and different concentrations of estrogen（0,10-12,10-11,10-10 and 10-9 mol/L） were added,and measured PGES and PGFS mRNA expression by using Real-time PCR.The results showed that different concentrations of E2 or different stimulation time in the same concentration of E2 could increase the expression of prostaglandin E2 synthase（PGES）,but in addition of E2 4 h and the concentration was 10-10 mol/L compared with the control was extremly significant different（P＆lt;0.01）,while PGFS in addition of E2 24 h and the concentration was 10-12 mol/L compared with the control was extremly significant different（P＆lt;0.01）.Experimental results showed that estrogen could promote the expression of PGES and PGFS mRNA in oviduct epithelial cells of bovine,and regulate the mRNA expression of prostaglandin synthase PGES and PGFS.

关 键 词：雌激素 输卵管上皮细胞 PGES PGFS 荧光定量PCR 

分 类 号：Q579.1[生物学—生物化学]