猪乙型脑炎病毒LS株的理化特性及其在BHK-21细胞上增殖特性的研究  

Study on the Physicochemical Properties and Proliferative Characteristics in BHK-21 Cells of Japanese Encephalitis Virus LS Strain

作　　者：王帅涛[1] 李伟豪[1] 王川庆[1] 刘红英[1] 王新卫[1] 常洪涛[1] 魏亚鹏[1] 

机构地区：[1]河南农业大学牧医工程学院,河南郑州450002

出　　处：《中国畜牧兽医》2013年第3期134-136,共3页China Animal Husbandry & Veterinary Medicine

摘　　要：对猪乙型脑炎病毒(Japanese encephalitis virus,JEV)LS株的理化特性及其在BHK-21细胞上的增殖特性进行研究,结果表明,该病毒对温度(56℃)、酸(pH 5.0以下)、乙醚、胰蛋白酶敏感,反复冻融(-65～20℃)3次几乎不影响病毒效价;该毒株在BHK-21细胞上连续传20代,仍能维持较高的病毒滴度(TCID50=107.75/mL)和血凝效价(28),且根据其在BHK-21细胞上的增殖规律,得到最佳收毒时间为接毒后28～40h。本试验为进一步研究JEV LS株的生物学特性奠定基础。The physicochemical properties and proliferative characteristics in BHK-21 cells of Japanese encephalitis virus（JEV） LS strain were studied in the test.The results indicated that JEV LS strain was sensitive for temperature（56 ℃）,acid（below pH 5.0）,diethyl ether and trypsin.The viral titer of LS strain remained at the same level undergoing a repeated freeze-thaw test.The TCID50 and hemagglutination titer of LS strain kept at the high level（TCID50=107.75/mL,28） after 20 serial passages in BHK-21 cells.According to the proliferation dynamic curve of JEV LS strain in BHK-21 cells,the optimum time to harvest JEV was 28 to 40 h after virus innoculation.This research laid a foundation for the further research on biological characteristics of JEV LS strain.

关 键 词：乙型脑炎病毒 理化特性 增殖规律 

分 类 号：S852.65[农业科学—基础兽医学]