土槿皮乙酸B通过活性氧和有丝分裂原蛋白激酶诱导MCF-7细胞凋亡的研究  

Study of Pseudolaric Acid B Induced Apoptosis through ROS Regulating MAPK in MCF-7 Cells

作　　者：刘春禹 郑桂彬[2] 于静华[3] 

机构地区：[1]吉林省中医院针灸科,吉林长春130000 [2]吉林大学白求恩第一医院甲状腺外科,吉林长春130000 [3]吉林大学白求恩第一医院艾滋病与病毒研究所,吉林长春130000

出　　处：《中国畜牧兽医》2013年第3期146-149,共4页China Animal Husbandry & Veterinary Medicine

基　　金：吉林省中医药管理局(2010-pt064);吉林省中医药管理局(2012-030)

摘　　要：土槿皮乙酸B(PAB)通过活性氧诱导肿瘤细胞MCF-7凋亡,同时也通过调控有丝分裂原蛋白激酶(MAPK)家族蛋白来调控凋亡,但活性氧和MAPK的关系还不清楚,本研究探讨活性氧和MAPK家族蛋白的关系。4μmol/L PAB在36h诱导细胞死亡,活性氧清除剂抑制PAB的作用,并降低PAB的抑制率;同时,PAB促进JNK磷酸化,抑制ERK的磷酸化,此作用被活性氧清除剂抑制。The assay was aimed to study the relationship of ROS and MAPK family protein in pseudolaric acid B（PAB）-induced MCF-7 cell apoptosis.Contrast phase microscope was used to observe cellular morphologic alternation;MTT analysis detected the inhibitory ratio;flow cytometric analysis of DCFH-DA staining determined the level of ROS,and Western blotting detected the protein expression.We found that PAB induced MCF-7 cell apoptosis through increasing ROS and increased the phosphoration of JNK and decrease the phosphoration of ERK.

关 键 词：土槿皮乙酸B 活性氧 凋亡 MAPK家族蛋白 人乳腺癌MCF-7细胞 

分 类 号：Q25[生物学—细胞生物学]