牛源耐甲氧西林金黄色葡萄球菌的检测  

Detection of Methicillin-resistant Staphylococcus aureus Strains Isolated from Bovine Mastitis

作　　者：苏洋[1,2] 蒲万霞[2] 邓海平[2] 李春慧[1,2] 梁红雁[1,2] 陈智华[1] 

机构地区：[1]甘肃农业大学动物医学院,甘肃兰州730070 [2]中国农业科学院兰州畜牧与兽药研究所,农业部兽用药物创制重点实验室,甘肃省新兽药工程重点实验室,甘肃兰州730050

出　　处：《中国畜牧兽医》2013年第3期181-184,共4页China Animal Husbandry & Veterinary Medicine

基　　金：中央级公益性科研院所基本科研业务费专项资金项目(1610322011010);中国农业科学院科技经费项目

摘　　要：本研究旨在了解甘肃地区奶牛乳房炎金黄色葡萄球菌的耐药性和耐甲氧西林金黄色葡萄球菌的感染情况,为奶牛乳房炎的防制提供理论依据。采用KB纸片扩散法,检测17株金黄色葡萄球菌对8种不同抗菌药物的敏感性;再用琼脂稀释法检测了苯唑西林、万古霉素对金黄色葡萄球菌的最小抑菌浓度(MICs);头孢西丁纸片扩散法和PCR扩增特异性mecA耐药基因对所有受试菌株进行全面的耐甲氧西林金黄色葡萄球菌检测。结果表明,菌株对青霉素、磺胺异恶唑具有较强抗性,而对环丙沙星、头孢唑啉、万古霉素和苯唑西林全敏感;头孢西丁纸片扩散法未能检测出表型为MRSA的阳性菌株,而PCR方法却检测出8株mecA基因阳性菌株,且这些菌株的苯唑西林MIC均小于2μg/mL。菌株的耐药情况较严重,对甲氧西林敏感而携带mecA基因的菌株高频存在于被调查地区的奶牛场中。The aim of present study was to investigate the antimicrobial resistance,and the prevalence of methicillin-resistant Staphylococcus aureus（S.aureus） isolated from bovine mastitis in Gansu province,to provide credible theory evidence for prevention and treatment on bovine mastitis.Eight commonly used antimicrobial agents were used for determining antimicrobial susceptibility of 17 total S.aureus strains by disk diffusion method.Agar screen method was used for determining the oxacillin and vancomycin minimum inhibitory concentration value as well.Disk diffusion method using the cephamycin antibiotics cefoxitin and detection of mecA gene by PCR assay were performed to detect the presence of MRSA.Most of strains showed a high resistance for penicillin and sulfafurazole,yet keeping complete sensitivity for ciprofloxacin,cefazolin,vancomycin and oxacillin.None MRSA isolate was identified by the phenotypic detection method,but eight MRSA isolates with the MIC of oxacillin lower than 2 μg/mL were found to carry mecA gene via PCR approach.This study suggested that the antimicrobial resistance of S.aureus of bovine origin was very serious.High frequency of mecA-positive oxacillin-susceptible S.aureus（OS-MRSA） were present in our herds.

关 键 词：金黄色葡萄球菌 耐药性 耐甲氧西林金黄色葡萄球菌 MECA基因 

分 类 号：S852.611[农业科学—基础兽医学]