产ESBLs大肠埃希菌耐药性及基因型分析  

Drug resistance and genotype analysis of ESBL-producing Escherichia coli

作　　者：陈枫[1] 黄永茂[1] 曹勇[1] 唐小平[1] 林雁[1] 陈庄[1] 

机构地区：[1]泸州医学院附属医院,四川泸州646000

出　　处：《中国病原生物学杂志》2013年第2期123-125,共3页Journal of Pathogen Biology

基　　金：四川省重点学科重点建设项目(No.SZD0241)

摘　　要：目的了解川南地区医院感染产β-内酰胺酶(ESBLs)大肠埃希菌基因型特征和临床耐药情况。方法收集2010年10月～2011年9月期间本院住院患者分离的大肠埃希菌,双纸片协同试验确定产ESBLs大肠埃希菌,PCR扩增技术进行基因分型,K-B法比较产ESBLs阳性株与阴性株耐药率。结果 77株大肠埃希菌双纸片协同试验确认28为株产ESBLs菌株;PCR扩增显示,28株产ESBLs大肠埃希菌中TEM型11株(39.29%),SHV型6株(21.43%),TEM、SHV双基因型2株(7.14%),非TEM、非SHV型9株(32.14%)。K-B法测定ESBLs阳性株对多种抗生素的耐药率(亚胺培南除外)高于ESBLs阴性株。结论川南地区产ESBLs大肠埃希菌以TEM型为主,对常用抗菌药物耐药率高于ESBLs阴性菌株。Objective To investigate genotypes and clinical drug resistance of ESBL producing Escherichia coli in hospi-tals in southern Sichuan. Methods E. coli was collected from October 2010 to September 2011. ESBLs were detected using a double disk synergy test, PCR was used to determine the genotype of the ESBLs, and resistant strains that were positive and negative for ESBLs were tested by the Kirby-Bauer method. Results There were 28 strains of ESBL-pro-ducing E. coli, including 11 strains （39.29 %） producing TEM beta-lactamases, 6 strains （21.43%） producing SHV be-ta-lactamases, 2 strains （7. 14%） producing both TEM and SHV beta-lactamases, and 9 strains （32. 14%） producing neither TEM nor SHV beta-lactamases. ESBL-produeing E. coli had a significantly higher rate of multi-drug resistance （excluding imipenem） compared to strains that were ESBL-negative. Conclusion In southern Siehuan, ESBL-producing E. coli produced primarily TEM beta-lactamases. ESBL-producing E. coli had a significantly higher rate of resistance than strains that were ESBL-negative.

关 键 词：大肠埃希菌 超广谱Β-内酰胺酶 基因型 

分 类 号：R378.21[医药卫生—病原生物学]