正交设计在灯盏乙素提取优化中的应用  

Application of Orthogonal Optimization in Extracting Scutellarin

作　　者：熊华斌[1,2] 阿新祥[3] 杨欣雨[2] 陈毅坚[1,2] 陈孟生[2] 陈名红[1,2] 

机构地区：[1]云南民族大学民族药资源化学国家民委-教育部重点实验室,云南昆明650500 [2]云南民族大学化学与生物技术学院,云南昆明650500 [3]云南省农业科学院生物技术与种质资源研究所,云南昆明650223

出　　处：《北方园艺》2013年第6期164-166,共3页Northern Horticulture

基　　金：云南省教育厅科学研究基金重点计划资助项目(2010Z073);云南民族大学生物技术试验示范中心资助项目(0205-2001015209)

摘　　要：以灯盏花为原料,利用正交实验对灯盏乙素提取的工艺参数进行了优化研究。结果表明:料液比为1∶2、甲醇浓度为80%和超声时间为30min的提取方案最优,其精密度和重复性试验的相对标准偏差仅为0.19%和2.46%,加标回收率达到了99.88%。通过正交设计优化后的灯盏乙素提取方法具有较好的重复性和可行性。Taking Erigeron breviscapus （Vant.） Hand-Mazz as material, the extraction process of scutellarin was optimized by orthogonal experiment. The results showed that the most excellent technology was moderate ethanol concentration 80%, solid-liquid ratio 1 ： 2 and ultrasonic time 30 min. The RSD of precision and repeatability experiment were 0. 19% and 2. 46% respectively, and the recovery of standard addition was 99. 88%. Therefore, the extraction technology which optimized by the orth0gonaI experimental was better repeatability and reliability.

关 键 词：灯盏乙素 正交优化 紫外分光光度法 检验 

分 类 号：S567.239[农业科学—中草药栽培]