利用同步辐射硬X射线微聚焦线站研究纳米树状分子载药行为  

Investigation of drug loading behavior of dendrimers nano-vectors with synchrotron radiation hard X-ray fluorescence analysis

作　　者：王璐[1] 张元庆[2] 赵云[1] 孙艳红[2] 诸颖[2] 许晓平[2] 黄庆[2] 

机构地区：[1]四川大学生命科学学院成都610065 [2]中国科学院上海应用物理研究所嘉定园区上海201800

出　　处：《核技术》2013年第3期1-4,共4页Nuclear Techniques

基　　金：国家自然科学基金(批准号:U1232114,U1232113,11275251,11179004)资助

摘　　要：同步辐射光源具有非常高的灵敏度及空间分辨,使其在环境科学、纳米材料及生命科学中具有广阔的应用前景。本研究利用同步辐射光源硬X射线荧光成像技术研究纳米树状分子的聚酰胺-胺(polyamidoamine,PAMAM)的载药行为,首先对PAMAM表面的游离氨基进行乙酰化,然后进行Cu2装载,通过硬X射线荧光成像检测细胞内的Cu2研究PAMAM载带Cu2进入细胞的情况。结果显示,PAMAM能够增加金属离子或药物进入细胞内。本研究为利用同步辐射硬X射线荧光成像技术研究功能化修饰的PAMAM的靶向及多功能成像提供了思路。Background： Synchrotron Radiation X-ray fluorescence analysis is an advanced method of quantitative elemental analysis. It is a scientific platform for life science, material science, and environmental science. Purpose： In this present study, the drug loading behavior of dendrimers nano-vectors was investigated by using the method of Synchrotron Radiation X-ray fluorescence analysis. Methods： The copper（II） ions were loaded into acetyl-modified generation 5（G5） PAMAM dendrimers. We monitored and imaged the distribution of copper in A549 cell by Shanghai Synchrotron Radiation Facility （SSRF） to observe the cellular uptake of copper-dendrimers conjugates. Results： The results of hard X-ray fluorescence mapping showed a stronger fluorescence of Cu in the cells treated with G5-Ac-Cu than that of control. Conclusions： This investigation provided a method of using synchrotron radiation X-ray fluorescence analysis to study the drug loading behavior of dendrimers.

关 键 词：同步辐射 硬X射线荧光成像 树状分子 纳米载体 

分 类 号：Q331[生物学—遗传学]